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EC number: 701-197-2
CAS number: -
results of the mouse lymphoma assay are shown in Table 1. The assays
were first performed over a wide range of concentrations, then rerun
over the narrow ranges shown in the table to demonstrate dose response.
The relative growth column is an index of the amount of toxicity to the
cells. In most cases the range of concentrations was restricted to
5-fold or less due to toxicity. The mutation frequency was calculated by
dividing the number of colonies appearing in the selective plates (TFT)
by the number of surviving cells as determined by plating a sample of
cells in the absence of the selective agent. The mutation index was
calculated by dividing the mutation frequency of the test results by the
mutation frequency of the solvent control. For this particular set of
experiments, we considered a compound positive if dose-related increases
in the mutation index over at least 3 concentrations with the highest
response at least equal to 3.0 were obtained. The mouse lymphoma assays
were performed without metabolic activation, with metabolic activation
by non-induced rat liver microsome preparation, and with an
Aroclor-1254-induced rat liver microsome preparation.
general, the highest mutagenic responses were obtained in the assays
without metabolic activation. Responses obtained were slightly reduced
in the assays that used the non-induced S9 preparations, whereas much
lower responses were obtained with the Aroclor-induced S9 preparations.
Cell toxicity was reduced proportionally, i.e. the same dose of chemical
produced a more toxic response in the absence of any S9 and a less toxic
response in the presence of the S9.
1: Results of the L5178Y TK+/- mouse lymphoma mutagenicity assay for
glycidol and Butyl glycidyl ester ± S9 mix
Relative growth (percent of control)
Mutation frequency per 10exp6 survivors
Mutation index (ratio of test/control)
Butyl glycidyl ester
Without exogenous metabolic activation.
With Aroclor-induced S9 fraction.
With uninduced S9 fraction.
Positive control without S9 fraction was ethyl methanesulfonate, 620
µg/ml; with induced S9 fraction, 2-acetylaminofluorene, 100 µg/ml; with
uninduced S9 fraction, dimethylnitrosamine, 74 µg/ml.
Positive control with S9 fraction for this compound only was
benzo[a]pyrene, 3 µg/ml.
a mammalian gene mutation assay (Mouse lymphoma assay, similar to OECD
476). L5178Y cell cultures were exposed to the read-across substances
glycidol and butyl glycidyl ester for '1,2,3-propanetriol, glycidyl
ethers' at concentrations of 8, 15, 23, 30, 45, 60, 75, 94, 125, 187,
250 µg/ml (glycidol) or 84, 100, 130, 164, 200, 256, 300, 320, 400, 500,
640, 800 µg/ml with and without metabolic activation. The metabolic
activation system was either liver homogenates prepared from
Aroclor-1254-induced Sprague-Dawley rats or rats injected with corn oil
and butyl glycidyl ester were both tested up to cytotoxic
concentrations, i.e. the highest dose was set at twice the level that
killed 50% of the organisms in the toxicity assay.
controls (ethyl methanesulfonate, 620 µg/ml, –S9; 2-acetylaminofluorene,
100 µg/ml, + induced S9; dimethylnitrosamine, 74 µg/ml, + uninduced S9)
induced the appropriate responses. For both glycidol and butyl glycidyl
ester there was a concentration-related positive response as well as the
stipulated at least three-fold increase of the mutation frequency over
background without or with both available metabolic activation systems.
So it can be concluded that '1,2,3-propanetriol, glycidyl ethers' has to
be considered as mutagenic in the mouse lymphoma assay, too.
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