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Toxicological information

Acute Toxicity: dermal

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Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
other information
Study period:
1976
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1976
Report Date:
1976

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 402 (Acute Dermal Toxicity)
Deviations:
yes
Remarks:
: no pathology performed, lack of details on test substance
GLP compliance:
no
Test type:
standard acute method
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 2-Chlor-1-nitrobenzol
- Physical state: solid
- Analytical purity: no data

Test animals

Species:
rat
Strain:
Wistar
Sex:
male

Administration / exposure

Type of coverage:
occlusive
Vehicle:
polyethylene glycol
Details on dermal exposure:
TEST SITE
- Area of exposure: shaved back
- Type of wrap if used: covered by alu and plaster


REMOVAL OF TEST SUBSTANCE
- Washing (if done): rinsed with water and soap
- Time after start of exposure: 24 hours
Duration of exposure:
24 hours
Doses:
male rats: 250, 350, 500, 750, 1000, 1500 mg/kg bw
No. of animals per sex per dose:
10
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LD50
Effect level:
655 mg/kg bw
Mortality:
See details below

Any other information on results incl. tables

dosis  conc.  result   signs of intoxication  time of death
mg/kg   %     m /s /n     start   end

 250    25    1/10/10     18 h   14 d             4 d
 350    25    1/10/10     18 h    7 d            18 h       
  
 500    50    3/10/10     18 h    9 d           1-3 d
 750    50    7/10/10     24 h   13 d           1-3 d 
1000    50    7/10/10     18 h    4 d        18 h - 3 d 
1500    75    9/10/10     18 h   14 d        18 h - 4 d

m:  number of rats which died; 
n: number of animals put in test
s: number of animals with signs of intoxication:
reduced general condition, difficulties in breathing,
cyanotic appearance, some animals showed lacrimation

Applicant's summary and conclusion