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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Comparable to guideline study with acceptable restrictions. Adopted according to OECD SIDS. The original source is available and has been reviewed.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Quantitative Structure-Activity Relationships of Nitroaromatic Compounds to Four Aquatic Organisms.
Author:
Zhao Y-H, Yuan X, Ji G-D, Sheng L-X, Wang L-S
Year:
1997
Bibliographic source:
Chemosphere 34 (8): 1837 - 1844
Reference Type:
secondary source
Title:
1-Chloro-2-nitrobenzene CAS: 88-73-3, SIDS Initial Assessment Report
Author:
OECD
Year:
2001
Bibliographic source:
UNEP publications

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Version / remarks:
(1984)
Deviations:
yes
Remarks:
: test duration 96 h instead of 72 h
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 2-Chloronitrobenzene
- Analytical purity: no data

Sampling and analysis

Analytical monitoring:
not specified

Test solutions

Vehicle:
yes
Details on test solutions:
- stock solutions were prepared in acteone

Test organisms

Test organisms (species):
other: algae: Scenedesmus obliquus

Study design

Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h

Test conditions

Test temperature:
24 ± 1 °C
Nominal and measured concentrations:
- 5 concentrations
- 3 replicates
Details on test conditions:
TEST SYSTEM
- Type: closed
- Size, fill volume: 100 mL, 50 mL
- Initial cells density: 10E4 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3


OTHER TEST CONDITIONS
- Photoperiod: 12 : 12 h
- Light intensity and quality: cool white fluorescent light, 4000 Lux (± 10%)


EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: spectrophotometer (at 650 nm)
- Observation intervals: daily
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
96 h
Dose descriptor:
EC50
Effect conc.:
18.1 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Executive summary:

Zhao (1997):

In a study similar to OECD 201 toxicity of 1-chloro-2-nitrobenzene to Scenedesmus obliquus was assessed. After 96 h of exposure an EC50 of 18.1 mg/L was determined based on growth rate.