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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo mammalian germ cell study: gene mutation
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable well documented report/publication meeting basic scientific principles.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1993
Reference Type:
publication
Title:
Unnamed
Year:
1985

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 477 (Genetic Toxicology: Sex-linked Recessive Lethal Test in Drosophila melanogaster)
Deviations:
yes
Principles of method if other than guideline:
Method: other: males(24 hrs old), mated with 3x with Basc virgin females brood1: 3d, brood2: 2d, brood3: 2d;
GLP compliance:
no
Type of assay:
Drosophila SLRL assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 2-Chloronitrobenzene
- Analytical purity: 99%
- Lot/batch No.: ET 00210KM
- Source: Aldrich Chemical Company (Milwaukee, WI)

Test animals

Species:
Drosophila melanogaster
Strain:
other: Canton-S wild type
Sex:
male
Details on test animals and environmental conditions:
- Chemical Analyses by Midwest Research Institute (MRI; Kansas City, MO)
- Karl Fischer analysis indicated less than 0.3% water
- Test substance: purity: 99 %

Administration / exposure

Route of administration:
oral: feed
Vehicle:
5% sucrose
Duration of treatment / exposure:
72 hours
Doses / concentrations
Remarks:
Doses / Concentrations:
0, 125 ppm in 10 % ethanol and 5 % sucrose solution
Basis:

No. of animals per sex per dose:
no data
Positive control(s):
no data

Examinations

Evaluation criteria:
no data
Statistics:
Normal approximation to the binomial test according to Margolin et al., 1983

Results and discussion

Test results
Sex:
male
Genotoxicity:
negative
Toxicity:
yes
Vehicle controls valid:
yes
Negative controls valid:
no
Positive controls valid:
no
Additional information on results:
Toxicity tests were performed to set concentrations at a level that would induce 30% mortality after 72 hours of feeding

Any other information on results incl. tables

Induction of sex-linked recessive lethal mutations in Drosophila melanogaster by 2-Chloronitrobenzene

Route of exposure

Dose (ppm)

Incidence Deaths (%)

Incidence Sterility (%)

No. of Lethals / No. of X chromosomes tested

Total1

Mating 1

Mating 2

Mating 3

Study performed at; data published in Zimmering et al. (1989)

Larva feeding

 

 

Larva feeding

 

59

0

 

60

0

1

 

 

10

0

 

 

0

1/1349

2/1341

 

3/1309

1/1281

1/1344

0/1332

 

1/1326

0/1234

 

2/2693 (0.07%)

2/2673 (0.07%)

 

4/2635 (0.15%)

1/2515 (0.04%)

Study performed at; data published in Zimmering et al. (1985)

Feeding

 

 

Injection

 

125

0

 

10000

0

8

 

 

15

9

 

 

9

4/3481

1/2282

 

0/3065

1/1455

1/1759

0/785

 

0/2635

1/1345

0/1847

1/520

 

1/2450

4/1160

5/7087 (0.07%)

2/3587 (0.06%)

 

1/8150 (0.01%)

6/3960 (0.15%)

1Total number of lethal mutations / total number of X chromosomes tested for up to three mating trials

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): negative