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Toxicological information

Skin irritation / corrosion

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Administrative data

Endpoint:
skin irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1976
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Comparable to guideline study with acceptable restrictions. Considering both, the application site (inside of the rabbit ear as a thin and sensitve skin area) and the prolonged exposure duration (24 h) the reported absence of skin reactions appears sufficient for assessment.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1976
Report Date:
1976

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 404 (Acute Dermal Irritation / Corrosion)
Deviations:
yes
Remarks:
only two animals used, application area inside of the animal ear, exposure duration 24 h instead of 4 h, no individual animal data (tabulation) given, lack of details on test substance
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 2-Chlor-1-nitrobenzol
- Analytical purity: no data

Test animals

Species:
rabbit
Strain:
other: albino
Details on test animals and environmental conditions:
Animal
-Weight: 3,5-4 kg
-Source: Gierlich, Bochum, Germany
-Housing: single
-Diet: Höing-Kaninchenfutter 222, Höing, Verden/Aller, Germany

Test system

Type of coverage:
semiocclusive
Preparation of test site:
other: hairless inside of the ear cup
Vehicle:
unchanged (no vehicle)
Controls:
no
Amount / concentration applied:
500 mg
Duration of treatment / exposure:
24 hour(s)
Observation period:
7 days
Number of animals:
2
Details on study design:
TEST SITE
- Area of exposure: a 2 x2 cm area of the animal ear
- Cellulose pads were loaded with 500 mg 1 -chloro-2 -nitrobenzene.
- Type of wrap if used: The test areas were covered semi-occlusive with a layer of cellulose and plaster.

REMOVAL OF TEST SUBSTANCE
no data

SCORING SYSTEM:
no data

Results and discussion

Any other information on results incl. tables

No scoring data mentioned

Applicant's summary and conclusion

Interpretation of results:
not irritating
Remarks:
Migrated information no classification required. Criteria used for interpretation of results: EU
Executive summary:

Groening (Bayer AG), 1976

The skin irritation potential of 1 -chloro-2 -nitrobenzene was investigated in albino rabbits. A 2 x 2 cm area of the animal ear inside was used to applicate the test substance. Cellulose pads were loaded with 500 mg 1 -chloro-2 -nitrobenzene. The test areas were covered semi-occlusively with a layer of cellulose and plaster. The exposure duration was 24 hours.

No irritating effects were observed throughout the test and after 7 days of observation.