Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 287-476-5 | CAS number: 85535-84-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 28 to 30 July 1982
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Well reported, good quality study to GLP; considered adequate for assessment
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Referenceopen allclose all
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 983
- Report date:
- 1983
- Reference Type:
- secondary source
- Title:
- Unnamed
- Year:
- 2 000
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Immobilisation of the freshwater crustacean, Daphnia magna, assessed following exposure to Chlorowax 500C (a C10-12 chlorinated paraffin; 58% chlorination) for 48 h.
- GLP compliance:
- yes
Test material
- Reference substance name:
- Alkanes, C10-13, chloro
- EC Number:
- 287-476-5
- EC Name:
- Alkanes, C10-13, chloro
- Cas Number:
- 85535-84-8
- IUPAC Name:
- Alkanes, C10-C13, Chloro
- Details on test material:
- - Name of test material (as cited in study report): Chlorowax 500C
- Substance type: technical product
- Physical state: liquid
- Analytical purity: "100% chlorinated paraffin"
- Impurities (identity and concentrations): free HCl, 7 ppm; no stabiliser
- Composition of test material, percentage of components: C10-12 chlorinated paraffin (58% chlorination)
- Lot/batch No.: R201-198
- Expiration date of the lot/batch: "dependent on routine analysis"
- Radiochemical purity (if radiolabelling): >98%
- Specific activity (if radiolabelling): 47 mCi/mmol
- Locations of the label (if radiolabelling): chlorinated n-undecane-6-14C
- Expiration date of radiochemical substance (if radiolabelling): no data
- Stability under test conditions: unstable
- Storage condition of test material: -20 degrees C
Constituent 1
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material (migrated information):
not relevant
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- Samples (3.0 mL) were taken of each test solution and analysed for C-14 concentration by liquid scintillation counting. At the end of the test, two replicates of each control and treatment were sampled and analysed in the same manner.
[On each occasion, samples were analysed from the control and solvent control to ensure that the count lay within 3 standard deviations of the background count for the scintillation counter].
Parent compound analysis was also conducted. At the start of the test, samples (250 ml) were taken from the control, 18, 100 and 560 ug/L nominal concentrations and extracted with hexane (30 ml) in an upwards displacement liquid/liquid extraction apparatus.
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- The chlorinated paraffin was mixed with the 14C-labelled chlorinated n-undecane by dissolving accurately weighed quantities of both compounds in acetone and mixing known volumes together. Individual stock solutions were prepared which, when diluted with water, would give the required total chlorinated paraffin concentration. The radioactivity in the test solutions was proportional to the total concentration of chlorinated paraffin. The minimum nominal activity of the solutions was 0.58 Bq/ml.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: freshwater flea
- Strain: Daphnia magna Straus
- Source: continuous laboratory cultures
- Age at study initiation (mean and range, SD): <24 h old
- Method of breeding: diploid parthenogenesis
- Feeding during test: no
ACCLIMATION
- Acclimation period: none
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Remarks on exposure duration:
- Assessments carried out after 24 and 48 h
- Post exposure observation period:
- none
Test conditions
- Hardness:
- 177 mg CaCO3/l
- Test temperature:
- 20+/-1 degree C (range 19.1-20.7 degree C, mean 19.8 degree C); temperature measured daily by thermometer and twice/h by automatic recorder in an additional vessel, identical to and situated amongst the test vessels, but containing only dilution water without Daphnia magna
- pH:
- control: pH 8.28 at start and pH 8.20-8.28 at the end of the test from 2 replicates
solvent control: pH 8.10 at start and pH 8.25-8.28 at the end of the test from 2 replicates
test solutions: pH 8.00-8.12 at start and pH 8.32-8.35 at the end of the test from 2 replicates - Dissolved oxygen:
- control: 8.80-9.15 mg/L at the end of the test from 2 replicates
solvent control: 8.60-8.65 mg/L at the end of the test from 2 replicates
test solutions: 8.60-9.20 mg/L at the end of the test from 2 replicates - Salinity:
- no data
- Nominal and measured concentrations:
- Nominal concentrations: 18, 32, 56, 100, 180, 320, 560 ug/L
Measured concentrations at 0 and replicate 48 h: 11-13, 22-26, 39-47, 68-82, 130-160, 240-270, 370-450 ug/L
Mean of the initial and final (mean of 2 replicates) measured concentrations: 12, 24, 43, 75, 145, 255, 415 ug/L (See Table 1 under "Any other information materials and methods incl. tables" below).
Control, solvent control and 18, 100 and 560 ug/L nominal test concentrations were also used to measure the test concentration by parent compound analysis. It was recognised that the method was relatively inaccurate but was the only available analytical technique specific for chlorinated paraffins. Concentrations of chlorinated paraffin measured as parent compound in the test solutions were <5, <5, 20, 100 and 600 ug/l respectively and by 14C analysis in the final hexane extracts were 13, 85 and 430 ug/l for the 18, 100 and 560 ug/L samples respectively. - Details on test conditions:
- TEST SYSTEM
- Test vessel: beakers
- Type (delete if not applicable): closed - loose fitting lids
- Material, size, headspace, fill volume: borosilicate glass beakers, 250 mL nominal capacity
- Aeration: >12 h before use
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate: no data
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: reconstituted water medium
- Total organic carbon: 0.9 mg/l
- Metals: cadmium 0.2 ug/l, copper 1.0 ug/l, cobalt <1.0 ug/l, iron 4.0 ug/l, lead <0.5 ug/l, manganese 0.8 ug/l, nickel <0.5 ug/l, zinc 2.0 ug/l
- Alkalinity: 122 mg/l
- Conductivity: 500 uS/cm
- Culture medium different from test medium: No
- Intervals of water quality measurement: No data
OTHER TEST CONDITIONS
- Photoperiod: 16 h light/8 h dark
EFFECT PARAMETERS MEASURED (with observation intervals if applicable): Immobilisation after 24 and 48 h (Each Daphnia magna was viewed by eye and was defined as immobile if showing no whole body movement relative to the water within a period of 10 seconds, even if movement of individual appendages was visible).
TEST CONCENTRATIONS
- Test concentrations (nominal): 0, 18, 32, 56, 100, 180, 320 and 560 ug/L
- Results used to determine the conditions for the definitive study: no data - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 530 µg/L
- Nominal / measured:
- estimated
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: 95% cCL 355-1540 ug/L
- Details on results:
- Flotation of Daphnia magna (either just below or on the surface) was observed in certain of the solutions containing the test substance and was most pronounced at nominal concentrations of 100 ug/L and above. After 24 h, no Daphnia were immobilised and all those floating were resubmerged with drops of the test solution. After 48 h, significant flotation had re-occurred at a nominal concentration of 180 ug/L and above and concentration related immobilisation was observed. Those Daphnia neither floating nor immobilised were observed to display somewhat erratic and reduced swimming activity at all concentrations except 18 ug/L (see Table 2 below for details)
- Results with reference substance (positive control):
- not applicable
- Reported statistics and error estimates:
- The values obtained for the 48 h EC50 for immobilisation were calculated by probit analysis and based on the mean measured concentrations of the test substance.
Any other information on results incl. tables
It was noted that the EC50 value was greater than the maximum measured concentration tested since the test substance caused the Daphnia magna to float, on or near the surface of the solution, at measured concentrations of approximately 75 ug/L and above.
Table 2. Percentage of immobolised and floating Daphnia at 24 and 48 h
Time (hours) |
Nominal concentration (ug/l) |
Total number tested |
% Immobilised |
% Floating |
24 |
control |
20 |
0 |
0 |
solvent control |
20 |
0 |
0 |
|
18 |
20 |
0 |
5 |
|
32 |
20 |
0 |
0 |
|
56 |
20 |
0 |
15 |
|
100 |
20 |
0 |
35 |
|
180 |
20 |
0 |
65 |
|
320 |
20 |
0 |
100 |
|
560 |
20 |
0 |
90 |
|
48 |
Control |
20 |
0 |
0 |
Solvent control |
20 |
0 |
0 |
|
18 |
20 |
0 |
0 |
|
32 |
20 |
0 |
0 |
|
56 |
20 |
0 |
0 |
|
100 |
20 |
5 |
5 |
|
180 |
20 |
5 |
45 |
|
320 |
20 |
25 |
20 |
|
560 |
20 |
40 |
5 |
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The 48 h EC50 for immobilisation of Chlorowax 500C (a C10-12 chlorinated paraffin; 58% chlorination) to the freshwater crustacean, Daphnia magna, was estimated to be 530 µg/L, based on measured concentrations.
- Executive summary:
In a well reported, good quality study to GLP, the acute toxicity of Chlorowax 500C (a C10-12 chlorinated paraffin; 58% chlorination) to the freshwater crustacean, Daphnia magna, was determined in a static system. Groups of 20 Daphnia, aged <24 h, were exposed to nominal concentrations of 0, 18, 32, 56, 100, 180, 320 or 560 µg/L for up to 48 h. The Daphnia were not fed during the course of the test and immobilisation was assessed after 24 and 48 h.
The 48 h EC50 for immobilisation to Daphnia magna was estimated, based on measured concentrations, to be 530 µg/L (95% CL 355-1540 µg/L). This value was in excess of the maximum measured concentration tested of 415 µg/L, since the test substance caused the Daphnia to float, on or near the surface of the solution, at measured concentrations of approximately 75 µg/L and above.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
