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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

biodegradation in soil
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Published study considered acceptable for assessment

Data source

Referenceopen allclose all

Reference Type:
Bacterial cometabolic degradation of chlorinated paraffins
Omori T, Kimura T and Kodama T
Bibliographic source:
Applied Microbiol. Biotechnol. 25: 553-557.
Reference Type:
secondary source

Materials and methods

Test guideline
no guideline available
Principles of method if other than guideline:
The degradation of Toyoparax 265 (a C12 chlorinated paraffin; 63% chlorination) was assessed by monitoring the release of chloride ion using a mixed bacterial culture isolated from soil.
GLP compliance:
not specified
Test type:

Test material

Constituent 1
Reference substance name:
Alkanes, C10-13, chloro
EC Number:
EC Name:
Alkanes, C10-13, chloro
Cas Number:
Alkanes, C10-C13, Chloro
Details on test material:
- Name of test material (as cited in study report): Toyoparax 265
- Substance type: technical product
- Physical state: no data
- Analytical purity: no data
- Composition of test material, percentage of components: C12 chlorinated paraffin (63% chlorinated; C12H18Cl8 )
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
no data

Study design

Oxygen conditions:
Soil classification:
not specified
Details on soil characteristics:
no data
Duration of test (contact time)
Soil No.:
ca. 48 h
Initial test substance concentration
Soil No.:
Initial conc.:
ca. 180 other: mg chlorine/1.2 L solution
Based on:
test mat.
Parameter followed for biodegradation estimation:
other: chloride ion release
Experimental conditions
Soil No.:
Details on experimental conditions:
Bacterial strains were isolated from soil using an enrichment culture containing n-hexadecane as the sole carbon source. The cometabolic biodegradation experiments were carried out with Toyoparax 265 (designated soil sample No. #1), with a concentration equivalent to 180 mg Cl/l.2 L solution) using a mixed bacterial inoculum (containing strains HK-3, HK-6, HK-8 and HK-10) incubated at 30oC for 48 hours.

Results and discussion

% Degradation
Soil No.:
% Degr.:
other: chloride release
Sampling time:
36 h
Transformation products:
not specified
Details on transformation products:
no data
Details on results:
Around 21% dechlorination of the test material was seen, with 32 µg/mL chloride being released
Results with reference substance:
The 4 bacterial strains (HK-3, HK-6, HK-8 and HK-10), grown on n-hexadecane, dechlorinated 1,10-dichlorodecane, 1-chlorobutane, 3-chlorobutyric acid and (for HK-10) 2-chloropropionic acid.

Applicant's summary and conclusion

Significant dechlorination of Toyoparax 265 (a C12 chlorinated paraffin; 63% chlorination) in a cometabolic process has been demonstrated. Around 21% dechlorination was seen after 36 hours with a co-culture of four soil derived bacteria in the presence n-hexadecane.
Executive summary:

Co-metabolic degradation studies were carried out with Toyoparax 265 (a C12 chlorinated paraffin; 63% chlorination), at a concentration of chloride equivalent to 180 mg/1.2 L solution, using a mixed bacterial inoculum (strains HK-3, HK-6, HK-8 and HK-10) at 30oC for 48 hours. The bacteria were isolated from soil using an enrichment culture containing n-hexadecane as sole carbon source.

Significant degradation was detected, with approx. 21% of the chloride being released after 36 hours, at which time bacterial growth and release of chloride ceased.