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Ecotoxicological information

Long-term toxicity to fish

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Administrative data

Endpoint:
adult fish: sub(lethal) effects
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Limited reporting in expert review; cited in final RAR (EU, 2000); considered acceptable for assessment.

Data source

Referenceopen allclose all

Reference Type:
review article or handbook
Title:
Unnamed
Year:
1975
Reference Type:
secondary source
Title:
Unnamed
Year:
2000

Materials and methods

Test guideline
Qualifier:
equivalent or similar to
Guideline:
OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
Deviations:
yes
Remarks:
incomplete reporting (no data on concentrations tested, no detailed results presented, only mininmal description of test methods)
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): C10-12 chlorinated paraffin
- Substance type: technical product
- Physical state: no data
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: C10-12 Chlorinated paraffin (58% chlorinated)
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
none

Sampling and analysis

Analytical monitoring:
not specified
Details on sampling:
no data

Test solutions

Vehicle:
not specified
Details on test solutions:
no data

Test organisms

Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
TEST ORGANISM
- Common name: Rainbow trout

Study design

Test type:
flow-through
Water media type:
not specified
Limit test:
no
Total exposure duration:
20 d
Post exposure observation period:
no data

Test conditions

Hardness:
no data
Test temperature:
10oC
pH:
no data
Dissolved oxygen:
no data
Salinity:
no data
Nominal and measured concentrations:
no data
Details on test conditions:
TEST CONCENTRATIONS
- Test concentrations: not clear from report, but appears to range between 0.04 and 100 mg/L
Reference substance (positive control):
not specified

Results and discussion

Effect concentrations
Duration:
20 d
Dose descriptor:
NOEC
Effect conc.:
< 0.04 mg/L
Nominal / measured:
not specified
Conc. based on:
test mat.
Basis for effect:
behaviour
Details on results:
Progressive loss of motor function leading to immobility within 15-20 days
Results with reference substance (positive control):
no data
Reported statistics and error estimates:
none

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified
Conclusions:
Exposure of rainbow trout (Onchorynchus mykiss) to a C10-12 chlorinated paraffin (58% chlorinated) at concentrations down to 0.04 mg/L resulted in progressive loss of motor function, and in some cases, death. The NOEC for this material was less than 0.04 mg/L. A LC50 of more than 100 mg/L after 24 days was noted.
Executive summary:

According to an expert review, Rainbow trout (Onchorynchus mykiss) were exposed to a C10-12 chlorinated paraffin (58% chlorinated) in a flow-through system at 10oC for up to 20 days. Although not clear from the report, it appears concentrations of between 0.04 and 100 mg/L were used. A LC50 of more than 100 mg/L after 24 days was noted. Various sub-lethal effects were reported resulting in a progressive loss of motor function and immobilisation, beginning within 15 to 20 days of the start of exposure. When death occured it resulted from debilitation, cessation of feeding and other secondary effects. The NOEC for the C10-12 chlorinated paraffin was less than 0.04 mg/L in this study.