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EC number: 287-476-5
CAS number: 85535-84-8
In a GLP study, conducted according to OECD
Guideline 474 (1983), Chlorowax 500C (a C10-13 chlorinated paraffin; 58%
chlorination) was assessed for its ability to cause chromosome damage in
the bone marrow erythrocytes of NMRI mice after oral exposure. Groups of
five mice of each sex were dosed by oral gavage with 5 g/kg bw of the
test compound in sesame oil and bone marrow erythrocytes were sampled
from the femurs after 24, 48 or 72 h. Further groups of mice (vehicle
controls) received sesame oil alone and samples were taken at the same
time points. The positive control, cyclophosphamide was given orally at
50 mg/kg bw and the bone marrow cells were sampled at 24 h. After slide
preparation and differential staining of the cells with
May-Grunwalds/Giema, 1000 cells each of polychromatic and mature
erythrocytes were counted and the number with micronuclei were recorded.
The ratio of polychromatic to mature erythrocytes was used to assess
No increase in the incidence of
micronucleated polychromatic erythrocytes or mataure erythrocytes was
evident in any of the treatment groups compared to the controls. The
ratio of polychromatic to mature erythrocytes was similar in treated and
untreated animals indicating that the test substance was not toxic to
the bone marrow cells. The positive control induced the expected
increase in micronuclei.
Under the conditions of the study, Chlorowax
500C showed no evidence of genotoxic activity in an in vivo bone marrow
micronucleus assay in male and female mice.
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