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EC number: 939-253-5
CAS number: 68424-85-1
Table 1. Results (based on active test
Test on natural soil
LC50 (based on emergence)
> 4990.0 mg/kg
[2772.2 – 4990.0]
EC50 (based on wet weight)
[359 – 1302]
[53 – 2185]
[151 – 632]
EC50 (based on dry weight)
[758 – 5066]
[187 – 1539]
[290 – 1390]
Test on quartz sand
[167 – 301]
[93 – 167] mg/kg
[93 – 301] mg/kg
[7 – 204]
[0 – 160]
[0 – 329]
[78 – 205]
[0 – 99]
[0 – 474]
The most sensitive was plant T. pratense
with EC50 = 634 mg/kg soil dry weight.
Conclusion: The effects of test substance exhibited great deviation
between sand and natural soil. In sand toxic effects take
place at lower concentrations than in soil, this is probably
due to the lower bioavailability of test substance in soil caused by
The test was considered as valid on the
basis of percent emergence and further growth of the plant in the water
The side-effects exhibited great deviation
between quartz sand and natural soil. The extraction of the active
substance proved that the natural soil had a strong sorbing effect, and
the total recovery was not achieved even when acidified methanol was
used. That was not the case with quartz sand, and this was taken as the
source of the differences: the availability of the active substance for
the plants differed, depending on the nature of the soil substrate.
A seedling emergence and seedling growth
test was conducted to determine the adverse effects of the test
substance (49.9% purity) on terrestrial plants according to OECD
Guideline 208, in compliance with GLP. Sinapis alba, Trifolium pratense
and Triticum aestivum were used. The substance was tested with 40 plants
per replicate per dose using 4 replicates for 16 days. The study was
performed using natural soil and sand at the following concentrations:
- Natural soil: 0, 476.6, 856.2, 1540.9,
2772.2 and 4990.0 mg/kg
- Sand: 0, 28.8, 55.8, 93.4, 166.8 and 300.5
Analytical determination of the test
substance was performed. The test was considered valid on the basis of
percent emergence and further growth of the plant in the water control.
Effects deviated between quartz sand and natural soil; in sand, toxic
effects were seen at lower concentrations than in soil. This was
probably due to the lower bioavailability of test substance in soil
caused by stronger adsorption. The extraction of the active substance
proved that the natural soil had a strong sorbing effect and total
recovery was not achieved even when acidified methanol was used as an
extraction solvent. That was not the case with quartz sand. The 16 day
EC50values in soil based on the effect on emergence and growth were 537,
634 and 1,960 mg a.i./kg dw for S. alba, T. pratense and T. aestivum,
respectively, while those in sand were 73, 74 and 141 mg a.i./kg dw,
respectively (Servajean E, 2004).
16 d EC50 values in soil based on the effect on emergence and growth
were 537, 634 and 1,960 mg a.i./kg dw for S. alba, T.
pratense and T. aestivum, respectively.
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