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EC number: 445-790-1
CAS number: 404362-22-7
The test material induced small but statistically significant increases
in the frequency of cells with aberrations in the 48 hour continuous
exposure group and in the 6(18)-hour exposure group. It should be noted
however, that in both cases the responses were within the historical
maxima for that time point, both were in comparison to very low vehicle
control values and were not part of any dose-related response.
Therefore, both increases were considered to be of no toxicological significance.
The test material did not induce any statistically significant increases
in the number of polyploid cells at any dose level in either treatment
This study was conducted according to a method which was designed to
assess the potential chromosomal mutagenicity of a test material on the
metaphase chromosomes of the Chinese Hamster Lung (CHL) cell line
according to OECD TG 473.
Duplicate cultures of Chinese Hamster Lung (CHI+) cells were treated
with the test material at several dose levels, together with vehicle and
positive controls. Five exposure groups were used: Experiment 1 included
a 6(18)-hour exposure, both with and without the addition of an induced
rat liver homogenate metabolising system; Experiment 2 included a
24-hour continuous exposure, a 48-hour continuous exposure and a repeat
of the 6(18)-hour exposure with metabolic activation.
The dose levels evaluated in the main experiments were selected from a
range of dose levels based on the results of a preliminary toxicity test
and were in the range of 3.75 to 120 µg/mL for the 6(18)-hour exposure,
both with and without S9, and 0.25 to 5 µg/mL for the 24 and 48-hour
The vehicle (solvent) controls gave frequencies of cells with
aberrations within the range expected for the CHL cell line. All the
positive control chemicals induced highly significant increases in the
frequency of cells with aberrations indicating the satisfactory
performance of the test and of the activity of the metabolising system.
The test material did not induce any toxicologically significant
increases in the frequency of cells with aberrations in any of the
exposure groups. The test material was shown to be toxic to CHL cells in
vitro and optimal levels of toxicity were achieved.
The test material was shown to be non-clastogenic to CHL cells in vitro.
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