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EC number: 445-790-1
CAS number: 404362-22-7
Based on nominal loading the 96-Hour LL50 value for rainbow trout is 4.0 mg/L loading rate WAF.
A Klimisch-1 -rated study was performed to assess the acute toxicity of
the test material to rainbow trout (Oncorhynchus mykiss). The
method followed that described in the OECD TG 203.
Following a preliminary range-finding test, fish were exposed, in groups
of ten, to Water Accommodated Fractions (WAF) of the test material over
a range of nominal loading rates of 1.0, 1.8,3.2, 5.6, and 10 mg/L for a
period of 96 hours under semi-static test conditions. The number of
mortalities and any sub-lethal effects of exposure in each test and
control vessel were determined 3 and 6 hours after the start of exposure
and then daily throughout the test until termination after 96 hours.
The test material concentration in the test loading rates was determined
by high performance liquid chromatography (HPLC). The test material gave
a chromatographic profile consisting of 4 peaks. Given the different
water solubilities and partition coefficients of the four components,
toxicity cannot be attributed to a single component or a mixture of
components but to the test material as a whole. The results therefore
were based on nominal loading rates WAF only.
The 96-Hour LL50 based on nominal loading rates was 4.0 mg/L loading
rate WAF with 95% confidence limits of 3.6 - 4.5 mg/L loading rate WAF.
The No Observed Effect Loading rate was 1.8 mg/L loading rate WAF.
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