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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

Based on nominal loading the 96-Hour LL50 value for rainbow trout is 4.0 mg/L loading rate WAF. 

Key value for chemical safety assessment

Additional information

A Klimisch-1 -rated study was performed to assess the acute toxicity of the test material to rainbow trout (Oncorhynchus mykiss). The method followed that described in the OECD TG 203.

Following a preliminary range-finding test, fish were exposed, in groups of ten, to Water Accommodated Fractions (WAF) of the test material over a range of nominal loading rates of 1.0, 1.8,3.2, 5.6, and 10 mg/L for a period of 96 hours under semi-static test conditions. The number of mortalities and any sub-lethal effects of exposure in each test and control vessel were determined 3 and 6 hours after the start of exposure and then daily throughout the test until termination after 96 hours.

The test material concentration in the test loading rates was determined by high performance liquid chromatography (HPLC). The test material gave a chromatographic profile consisting of 4 peaks. Given the different water solubilities and partition coefficients of the four components, toxicity cannot be attributed to a single component or a mixture of components but to the test material as a whole. The results therefore were based on nominal loading rates WAF only.

The 96-Hour LL50 based on nominal loading rates was 4.0 mg/L loading rate WAF with 95% confidence limits of 3.6 - 4.5 mg/L loading rate WAF. The No Observed Effect Loading rate was 1.8 mg/L loading rate WAF.