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Short-term toxicity to aquatic invertebrates

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Description of key information

Based on nominal test loading rates the 48-Hour EL50 value for Daphnia is 3.4 mg/L loading rate WAF. 

Key value for chemical safety assessment

Additional information

A study was performed to assess the acute toxicity of the test material to Daphnia magna. The method followed that described in the OECD TG 202.

Following preliminary range-finding tests, twenty daphnids (2 replicates of 10 animals) were exposed to Water Accommodated Fractions (WAFS) of the test material over a range of nominal loading rates of 0.10, 0.18, 0.32, 0.56, 1.0, 1.8, 3.2, 5.6 and 10 mg/L for 48 hours under static test conditions. The number of immobilised Daphnia were recorded after 24 and 48 hours.

The test material concentration in the test loading rates was determined by high performance liquid chromatography (HPLC). The test material gave a chromatographic profile consisting of 4 peaks. Given the different water solubilities and partition coefficients of the four components, toxicity cannot be attributed to a single component or a mixture of components but to the test material as a whole. The results therefore were based on nominal loading rates WAF only.

The 48-Hour EL50 for the test material to Daphnia magna based on nominal loading rates was 3.4 mg/L loading rate WAF with 95% confidence limits of 2.9 - 3.9 mg/L loading rate WAF. The No Observed Effect Loading rate was 1.8 mg/L loading rate WAF.