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EC number: 445-790-1
CAS number: 404362-22-7
The ready biodegradability of the submission item was studied under GLP
in a modified MITI test in accordance with OECD TG 301C. The degradation
was studied in parallel in three bottles by adding 30 mg test substance
and 10 mL activated sludge (3000 mg suspended matter/L, diluted to 900
mg/L by addition of basal medium) to 290 mL basal medium. In addition,
an inoculum control (1 bottle), a positive control (1 bottle with
aniline and activated sludge) and an inorganic control (1 bottle with
test item and purified water) were run. The test was performed during 28
days at a temperature of 25 ± 1 °C in a closed system oxygen consumption
measuring apparatus. The biodegradability of the substance was surveyed
by regularly checking the oxygen consumption in the testing bottles. No
oxygen consumption over that of the inoculum control was observed during
the testing period of 28 days in the three testing bottles containing
the test item showing that no biodegradation had occurred. This finding
was confirmed by analysis of dissolved organic carbon and by analysis of
the four components of the submission item by means of gas
chromatography. No decline in the concentrations of dissolved organic
carbon or test material was observed over the period of 28 days
confirming the stability of the test item in the testing system. The
positive control substance aniline was degraded by 59 % within 7 days,
which showed the appropriateness and normal activity of the applied
activated sludge. It was concluded that the submission item was not
biodegradable under the conditions of this modified MITI test.
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