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Short-term toxicity to aquatic invertebrates

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Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Analytical monitoring:
yes
Details on sampling:
- No data
Vehicle:
yes
Details on test solutions:
PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Method: Stock Solutions Preparations and Stability: Pipette or pour the appropriate amount of the solution (0.1wt% of test
chemical with solubilizer hydrogenated caster oil HCO-40, 4000 mg/L) into the test waters. Dilution Water Source: dechlorinated tap water.
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): hydrogenated caster oil HCO-40
- Concentration of vehicle in test medium (stock solution and final test solution): 100 mg/L
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no data
Test organisms (species):
Daphnia magna
Details on test organisms:
TEST ORGANISM
- Source: Supplied from U.S. EPA Environmental Research Laboratory
- Age at study initiation (mean and range, SD): <24 hours after hatching
Test type:
not specified
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
none
Hardness:
Dilution Water Chemistry: hardness = 55.6mg/L as CaCO3,
pH = 7.7, chlorine concentration <0.02 mg/L
Test temperature:
19-21 °C
pH:
7.7
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Type: no data whether open / closed
- Material, size, headspace, fill volume: 300 ml Petri dish (diameter = 8.5cm,depth = 5.7 cm )
- Aeration: no data
- Type of flow-through (e.g. peristaltic or proportional diluter):
- Renewal rate of test solution (frequency/flow rate):
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): 4
- Biomass loading rate: no data


TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dechlorinated tap water
- Chlorine: <0.02 mg/L



OTHER TEST CONDITIONS
- Adjustment of pH: no data
- Photoperiod: 16h:8h light-dark cycle
- Light intensity: room light


EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : immobility


TEST CONCENTRATIONS
- Spacing factor for test concentrations: 1.5
- Justification for using less concentrations than requested by guideline: n.a.
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
6.77 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 5.88-7.71 mg/L

Nominal/measured Concentration:

Measured concentration (mg/L)

(percentage of nominal)

Nominal

concentration

(mg/L)

0-hour

24-hour

Mean*

control

n.d.

n.d.

n.d

Solvent control

n.d.

n.d.

n.d.

4.94

5.29

(107)

3.54

(71.6)

4.35

(88.1)

7.41

8.0

(108)

5.41

(73.1)

6.63

(89.4)

11.1

11.7

(106)

8.16

(73.5)

9.83

(88.6)

16.7

16.7

(99.8)

13.3

(79.8)

14.9

(89.4)

25.0

23.7

(94.7)

20.0

(79.9)

21.8

(87.1)

Water Temperature: 20.2-20.3 °C

Water Chemistry in test: pH 7.6-7.8; Dissolved oxygen = 8.7-8.8 mg/L(Oxygen saturation level>=60%)

Cumulative immobility:

Cumulative number of Immobilized Daphnia

(percent immobility)

Measured

concentration

(mg/L)

24-hour

48-hour

control

0(0)

0(0)

Solvent control

0(0)

0(0)

4.35

0(0)

1(5)

6.63

3(15)

11(55)

9.83

14(70)

18(90)

14.9

18(90)

19(95)

21.8

19(95)

20(100)

Ei C50: 6.77 mg/L (48hr); 95% confidence limits: 5.88 -7.71 mg/L

NOECi: -

Ei C100: 21.8 mg/L (48hr)

Validity criteria fulfilled:
yes
Conclusions:
The acute toxicity to Daphnia was EC50 (48 h) = 6.77 mg/L
Executive summary:

The acute toxicity to Daphnia was EC50 (48 h) = 6.77 mg/L (95% confidence range 5.88- 7.71 mg/L) in an OECD TG 202 study that was conducted under GLP (CITI, 1997).

Description of key information

The acute toxicity to Daphnia was EC50 (48 h) = 6.77 mg/L (95% confidence range 5.88- 7.71 mg/L) in a guideline GLP study (OECD TG 202).

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
6.77 mg/L

Additional information

The acute toxicity to Daphnia was EC50 (48 h) = 6.77 mg/L (95% confidence range 5.88- 7.71 mg/L) in an OECD TG 202 study that was conducted under GLP (CITI, 1997).