3,5,5-trimethylhexan-1-ol

Long-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

adult fish: sub(lethal) effects

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

secondary literature

Remarks:

Only secondary literature available (SIDS documents; Iuclid reference [10]; Reliability assigned: 1)

Qualifier:

according to guideline

Guideline:

OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)

Deviations:

no

GLP compliance:

yes

Analytical monitoring:

yes

Vehicle:

yes

Test organisms (species):

Oryzias latipes

Test type:

flow-through

Water media type:

freshwater

Limit test:

no

Total exposure duration:

14 d

Duration:

14 d

Dose descriptor:

LC50

Effect conc.:

> 17 mg/L

Nominal / measured:

meas. (TWA)

Conc. based on:

test mat.

Basis for effect:

mortality

Duration:

14 d

Dose descriptor:

NOEC

Effect conc.:

1.28 mg/L

Conc. based on:

test mat.

Basis for effect:

behaviour

Nominal/measured Concentration:

	Measured concentration (mg/L)       (percentage of nominal)
Nominal concentration (mg/L)	0-day	7-day	14-day	Mean*
control	n.d.	n.d.	n.d	n.d.
Solvent control	n.d.	n.d.	n.d.	n.d.
0.512	0.499 (97.6)	0.449 (87.7)	0.480 (93.8)	0.476 (93.0)
1.28	1.25 (97.6)	1.10 (85.7)	1.23 (96.2)	1.19 (93.1)
3.20	3.09 (96.6)	2.59 (80.8)	2.87 (89.8)	2.85 (89.1)
8.00	7.80 (97.5)	7.02 (87.8)	7.31 (91.4)	7.32 (92.2)
20.0	17.6 (88.2)	16.7 (83.6)	17.1 (85.4)	17.1 (85.7)

* Expressed as arithmetic means calculated

Water Temperature: 23.9 - 24.3 degree C

Water Chemistry in test: pH 6.9-7.5; Dissolved oxygen = 7.6-8.3 mg/L(oxygen saturation level>=60%)

Mortality:

Cumulative mortality:

	cumulative number of dead fish mortality (%) vs time (day)
Nominal concentration (mg/L)	1	2	3	4	5	6	7	8	9	10	11	12	13	14
control	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)
Solvent control	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)
0.512	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)
1.28	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)
3.20	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)
8.00	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)
20.0	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	0 (0)	1 (10)	1 (10)	1 (10)

LC50, Exposure time 7days:       > 20mg/L

LC50, Exposure time 14days:    > 20mg/L

Other effects : Toxic symptoms

	Toxic symptoms, on day
Nominal concentration (mg/L)	1	2	3	4	5	6	7	8	9	10	11	12	13	14
control	-	-	-	-	-	-	-	-	-	-	-	-	-	-
Solvent control	-	-	-	-	-	-	-	-	-	-	-	-	-	-
0.512	-	-	-	-	-	-	-	-	-	-	-	-	-	-
1.28	-	-	-	-	-	-	-	-	-	-	-	-	-	-
3.20   AB	-	-	1	-	-	-	-	-	-	-	-	-	-	-
3.20 RFA	-	5	3	-	-	-	-	-	-	-	-	-	-	-
8.00   AB	2	2	10	8	5	5	5	5	5	5	4	2	-	-
8.00   RFA	10	10	10	10	10	10	10	10	10	10	10	10	10	10
20.0   AB	10	10	10	10	10	10	10	10	10	10	10	9	9	9
20.0   RFA	10	10	10	10	10	10	10	10	10	10	10	9	9	9
20.0   LT								-	-	-	4	3	3	3

The values express the number of fish showing the symptoms:

AB : Abnormal behavior; RFA: Reduced feeding activity; LT : Light body color; - : no symptom

Effect levels:

-NOEC(14 d):              1.28 mg/L

-EC50 (behavior):        3.20 (2.17-4.72) mg/L

Validity criteria fulfilled:

yes

Conclusions:

Key results in a 14-day fish toxicity test were as follows:
- the LC50 (14d) was > 20 mg/L
- behavioral changes were seen in approx. 50% of the fish at nominal 8 mg/L, during days 1 through day 14
- Effect levels:
-NOEC(14 d):              1.28 mg/L
-EC50 (behavior):        3.20 (2.17-4.72) mg/L

Executive summary:

3,5,5,-trimethylhexan-1 -ol was examined in a 14-day fish toxicity test according to OECD TG 204 and GLP. The nominal concentration ranged from 0.512 to 20 mg/L (measured approx. 0.475 - 17.1 mg/L). The key results were as follows:

- the LC50 (14d) was > 20 mg/L (nominal)

- behavioral changes were seen in approx. 50% of the fish at nominal 8 mg/L (measured mean 7.36 mg/L) during days 1 through day 14, and in all fish at nominal 20 mg/L

Effect levels:

- NOEC(14 d):               1.28 mg/L

- EC50 (behavior):        3.20 (2.17-4.72) mg/L

- LC50 (14d) > 20 mg/L

(source: CITI, 1997).

Description of key information

3,5,5,-trimethylhexan-1-ol was examined in a 14-day fish toxicity test according to OECD TG 204 and GLP. The NOEC value was 1.28 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

1.28 mg/L