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Biodegradation in water: screening tests

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Reference
Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
Database of the Japanese National Institute of Technology and Evaluation. Information on the chemical published in the Official Bulletin of Economy,Trade and Industry (Former title:The Official Bulletin of the Ministry of International Trade and Industry (published before Jan.6, 2001)). Original reference received in 2012: GLP guideline study.
Reason / purpose:
reference to same study
Qualifier:
according to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
GLP compliance:
yes
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
- Concentration of sludge: 30 mg/L
Duration of test (contact time):
4 wk
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
TOC removal
Parameter followed for biodegradation estimation:
test mat. analysis
Remarks:
gas chromatography
Details on study design:
TEST CONDITIONS
- Test temperature: 25°C


TEST SYSTEM
- Volume of test solution: 300 mL


SAMPLING
- Other: no data


CONTROL AND BLANK SYSTEM
- Other: no data



STATISTICAL METHODS: calculation of means of 3 replicate experiments
Parameter:
other: BOD
Value:
3.67
St. dev.:
1.15
Sampling time:
4 wk
Parameter:
% degradation (TOC removal)
Value:
4
St. dev.:
4
Sampling time:
4 wk
Details on results:
BOD and TOC data represent means +/- SD of 3 determinations
Parameter:
BOD5
Value:
ca. 3.67 other: %

 

Results percentage biodegradation(%)

average

BOD

5

3

3

4

TOC

0

4

8

4

GC

56

49

59

55

 

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
Biodegradation of 3,5,5 -trimethylhexan-1-ol was approx. 4% within 4 days, i.e. the test substance was not readily biodegradable
Executive summary:
3,5,5 -trimethylhexan-1-ol was not readily biodegradable in the MITI-I test (i.e. OECD 301C). Biodegradation was <4% BOD after 28 days of incubation. It was demonstrated that approx. 55% of the test substance was oxidised to 3,5,5,-trimethylhexanoic acid which was not further degraded within the 28 days of incubation (CERI 2007; NITE; 2002).

Description of key information

3,5,5-trimethylhexan-1-ol was not readily biodegradable in the MITI-I test (i.e. OECD 301C)

Key value for chemical safety assessment

Biodegradation in water:
under test conditions no biodegradation observed

Additional information

3,5,5 -trimethylhexan-1-ol was not readily biodegradable in the MITI-I test (i.e. OECD 301C). Biodegradation was <4% BOD after 28 days of incubation. It was demonstrated that approx. 55% of the test substance was oxidised to 3,5,5,-trimethylhexanoic acid which was not further degraded within the 28 days of incubation (CERI 2007; NITE; 2002).