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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
Only secondary literature available (SIDS documents; Iuclid reference [9]; Reliability assigned: 1)
Qualifier:
according to
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
yes
Test organisms (species):
Oryzias latipes
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
16.6 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
27.7 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 95% CL 16.-37.1 mg/L
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
37.1 mg/L
Nominal / measured:
meas. (TWA)
Conc. based on:
test mat.
Basis for effect:
mortality

Exposure concentrations

Nominal/measured concentration:       0 hr; 93.4 - 99.0 %,

24hr (water renewal):                    65.0 - 89.4%.

Nominal concentrations:

Nominal                Measured concentration (mg/L)

concentration       (percentage of nominal)

(mg/L)                   0-hours                  24-hours               Mean*

----------------- ----------------------------------------------------------------------

Control                  n.d.                        n.d.                         n.d.

Solvent Control       n.d.                        n.d.                         n.d.

7.90                      7.82                       5.26                       6.46

(99.0%)                 (66.6%)                (81.8%)

11.9                       11.2                       7.74                       9.36

(94.0%)                 (65.0%)                  (78.6%)

17.8                       17.3                       15.9                       16.6

(97.2%)                 (89.4%)                 (93.3%)

26.7                       24.9                       23.4                       24.2

(93.4%)                 (87.8%)                   (90.6%)

40.0                       38.8                       35.4                       37.1

               (96.9%)                 (88.6%)                   (92.7%)

----------------- ----------------------------------------------------------------------

* The values are expressed as time-weight calculated.

Water Temperature: 24.0-24.3 degree C

Water Chemistry in test: PH 7.0-7.5; DO = 5.8-8.3 mg/ L (Oxygen saturation level>=60%)

Cumulative mortality:

Measured                            Cumulative number of dead fish (Percent mortality)

concentration

(mg/L)                                   24-hr              48-hr            72-hr               96-hr

--------------- ----------------------------------------------------------------------------------------------------------------

Control                                   0(0)               0(0)             0(0)                  0(0)

Solvent control                          0(0)               0(0)             0(0)                   0(0)

6.46                                      0(0)               0(0)             0(0)                  0(0)

9.36                                     0(0)               0(0)             0(0)                  0(0)

16.6                                      0(0)               0(0)             0(0)                  0(0)

24.2                                      1(10)             1(10)        1(10)               2(20)

37.1                                    10(100)        10(100) 10(100) 10(100)

--------------- --------------------------------------------------------------------------------------------------------------- -

LC50 27.7 mg/L (95%confidence limits 16.6-37.1); Statistical method : binomial

LC0 16.6mg/L; LC100 37.1mg/L

Other effects : Toxic symptoms

Measured                                   Toxic symptoms

concentration

(mg/L)                   3 -hour       24-hour       48-hour       72-hour       96-hour

-------------- ----------------------------------------------------------------------------------------------------------------

Control                     -              -               -                   -              -

Solvent control           -              -               -                   -              -

6.46                        -          AB(10)       AB(10)        AB(10)       AB(8)

9.36                      -        AB(10)       AB(10)        AB(10)       AB(10)

LT(1)          LT(1)             LT(1)

16.6                     AB(6)       AB(10) AB(10) AB(10) AB(10)

LT(1)          LT(1) LT(1)

24.2                     AB(5)       AB(9)       AB(9)        AB(9)       AB(6)

      LT(2)        LT(4)               LT(5)       LT(7)

IM(5)                         IM(5)

37.1  -         n               n               n                 n

--------------- ----------------------------------------------------------------------------------------------------------------

The values in parentheses express the number of fish showing the symptom.

AB : Abnormal behavior

LT : Light body color

IM : Immobility

- : No symptom

n : No observation was made because all fish died at this observation time

Conclusions:
The LC50 was 27.7 mg/L (95% CL 16.6 - 37.1) in an acute fish toxicity test
Executive summary:

An acute fish toxicity test using a fresh water fish (Oryzias latipes) was conducted according to OECD TG 203 and under GLP. The LC50 (96 h) was 27.7 mg/L, based on measured concentrations (CITI, 1997).

Description of key information

Acute toxicity to fish: an acute fish toxicity test using a fresh water fish (Oryzias latipes) was conducted according to OECD TG 203 and under GLP. The LC50 (96 h) was 27.7 mg/L, based on measured concentrations (CERI, 1997).

Key value for chemical safety assessment

LC50 for freshwater fish:
27.7 mg/L

Additional information

Acute toxicity to fish: an acute fish toxicity test using a fresh water fish (Oryzias latipes) was conducted according to OECD TG 203 and under GLP. The LC50 (96 h) was 27.7 mg/L, based on measured concentrations. The value is contained in the approved SIDS document, but the original reference is not yet available (CERI, 1997). Results of model calculations give a lower value of LC50 (96 h) = 9.7 mg/L (GUBDH, 2008).

3,5,5,-trimethylhexan-1 -ol was also examined in a 14-day fish toxicity test according to OECD TG 204 and GLP in the same species ( Oryzias latipes). The LC50 (14d) was > 17 mg/L (nominal; CITI, 1997).