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EC number: 222-376-7 | CAS number: 3452-97-9
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Remarks:
- Only secondary literature available (SIDS documents; Iuclid reference [9]; Reliability assigned: 1)
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- yes
- Test organisms (species):
- Oryzias latipes
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 16.6 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 27.7 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 95% CL 16.-37.1 mg/L
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 37.1 mg/L
- Nominal / measured:
- meas. (TWA)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Sublethal observations / clinical signs:
Exposure concentrations
Nominal/measured concentration: 0 hr; 93.4 - 99.0 %,
24hr (water renewal): 65.0 - 89.4%.
Nominal concentrations:
Nominal Measured concentration (mg/L)
concentration (percentage of nominal)
(mg/L) 0-hours 24-hours Mean*
----------------- ----------------------------------------------------------------------
Control n.d. n.d. n.d.
Solvent Control n.d. n.d. n.d.
7.90 7.82 5.26 6.46
(99.0%) (66.6%) (81.8%)
11.9 11.2 7.74 9.36
(94.0%) (65.0%) (78.6%)
17.8 17.3 15.9 16.6
(97.2%) (89.4%) (93.3%)
26.7 24.9 23.4 24.2
(93.4%) (87.8%) (90.6%)
40.0 38.8 35.4 37.1
(96.9%) (88.6%) (92.7%)
----------------- ----------------------------------------------------------------------
* The values are expressed as time-weight calculated.
Water Temperature: 24.0-24.3 degree C
Water Chemistry in test: PH 7.0-7.5; DO = 5.8-8.3 mg/ L (Oxygen saturation level>=60%)
Cumulative mortality:
Measured Cumulative number of dead fish (Percent mortality)
concentration
(mg/L) 24-hr 48-hr 72-hr 96-hr
--------------- ----------------------------------------------------------------------------------------------------------------
Control 0(0) 0(0) 0(0) 0(0)
Solvent control 0(0) 0(0) 0(0) 0(0)
6.46 0(0) 0(0) 0(0) 0(0)
9.36 0(0) 0(0) 0(0) 0(0)
16.6 0(0) 0(0) 0(0) 0(0)
24.2 1(10) 1(10) 1(10) 2(20)
37.1 10(100) 10(100) 10(100) 10(100)
--------------- --------------------------------------------------------------------------------------------------------------- -
LC50 27.7 mg/L (95%confidence limits 16.6-37.1); Statistical method : binomial
LC0 16.6mg/L; LC100 37.1mg/L
Other effects : Toxic symptoms
Measured Toxic symptoms
concentration
(mg/L) 3 -hour 24-hour 48-hour 72-hour 96-hour
-------------- ----------------------------------------------------------------------------------------------------------------
Control - - - - -
Solvent control - - - - -
6.46 - AB(10) AB(10) AB(10) AB(8)
9.36 - AB(10) AB(10) AB(10) AB(10)
LT(1) LT(1) LT(1)
16.6 AB(6) AB(10) AB(10) AB(10) AB(10)
LT(1) LT(1) LT(1)
24.2 AB(5) AB(9) AB(9) AB(9) AB(6)
LT(2) LT(4) LT(5) LT(7)
IM(5) IM(5)
37.1 - n n n n
--------------- ----------------------------------------------------------------------------------------------------------------
The values in parentheses express the number of fish showing the symptom.
AB : Abnormal behavior
LT : Light body color
IM : Immobility
- : No symptom
n : No observation was made because all fish died at this observation time
- Conclusions:
- The LC50 was 27.7 mg/L (95% CL 16.6 - 37.1) in an acute fish toxicity test
- Executive summary:
An acute fish toxicity test using a fresh water fish (Oryzias latipes) was conducted according to OECD TG 203 and under GLP. The LC50 (96 h) was 27.7 mg/L, based on measured concentrations (CITI, 1997).
Reference
Description of key information
Acute toxicity to fish: an acute fish toxicity test using a fresh water fish (Oryzias latipes) was conducted according to OECD TG 203 and under GLP. The LC50 (96 h) was 27.7 mg/L, based on measured concentrations (CERI, 1997).
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 27.7 mg/L
Additional information
Acute toxicity to fish: an acute fish toxicity test using a fresh water fish (Oryzias latipes) was conducted according to OECD TG 203 and under GLP. The LC50 (96 h) was 27.7 mg/L, based on measured concentrations. The value is contained in the approved SIDS document, but the original reference is not yet available (CERI, 1997). Results of model calculations give a lower value of LC50 (96 h) = 9.7 mg/L (GUBDH, 2008).
3,5,5,-trimethylhexan-1 -ol was also examined in a 14-day fish toxicity test according to OECD TG 204 and GLP in the same species ( Oryzias latipes). The LC50 (14d) was > 17 mg/L (nominal; CITI, 1997).
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