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Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from publication.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
monograph
Author:
Opdyke et al.
Year:
1982
Bibliographic source:
Food and Chemical Toxicology
Reference Type:
other: authoritative database
Title:
Acute inhalation toxicity study
Author:
IFA GESTIS
Year:
2017
Bibliographic source:
GESTIS SUBSTANCE Database, 2017.
Reference Type:
other: authoritative database
Title:
Acute inhalation toxicity study in rat
Author:
Jay A. Brown
Year:
2017
Bibliographic source:
Haz-Map®

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
other: As mentioned below
Principles of method if other than guideline:
Acute inhalation toxicity study of test chemical in rat.
GLP compliance:
not specified
Test type:
other: not specified
Limit test:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Name: 2-MethoxyphenolInChI:1S/C7H8O2/c1-9-7-5-3-2-4-6(7)8/h2-5,8H,1H3Smiles: COc1ccccc1O- Name of test material:Guaiacol- Molecular formula :C7H8O2- Molecular weight :124.1382 g/mol- Substance type:organic- Physical state:Colorless solid

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified
Details on test animals and environmental conditions:
not specified

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
not specified
Vehicle:
not specified
Remark on MMAD/GSD:
not specified
Details on inhalation exposure:
not specified
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
2 h
Remarks on duration:
not specified
Concentrations:
Range between 2,000 and 17,000 mg/m³
No. of animals per sex per dose:
not specified
Control animals:
not specified
Details on study design:
- Necropsy of survivors performed: yes- Other examinations performed: Animals were observed for mortality and clinical signs.
Statistics:
not specified

Results and discussion

Preliminary study:
not specified
Effect levels
Sex:
not specified
Dose descriptor:
LC50
Effect level:
7 570 mg/m³ air
Based on:
test mat.
Exp. duration:
2 h
Remarks on result:
other: 50% mortality was observed
Mortality:
50% mortality was observed
Clinical signs:
Initially all animals exhibited slight inflammations of the eyes and the respiratory tract as well as agitation, followed by a loss of activity. After exposures to values up to 4,000 mg/m³ these symptoms were reversible after the termination of the exposure. Higher concentrations caused further CNS and neuromuscular symptoms (weak reflexes, unsteady gait, respiratory disorders, tonic-clonic spasms).
Body weight:
not specified
Gross pathology:
The dissection revealed injuries in several organs (vascularisation, bleeding, dystrophic changes).
Other findings:
not specified

Applicant's summary and conclusion

Interpretation of results:
other: Not classified
Conclusions:
The acute inhalation toxicity dose (LC50) was considered to be 7570 mg/m³, when mice were exposed to test chemical via inhalation by vapor for 2 hour exposure.
Executive summary:

The acute inhalation toxicity study was conducted by using test chemical in mice at the concentration range between 2,000 and 17,000 mg/m³. Animals were observed for mortality and clinical signs. Necropsy was performed. 50% mortality was observed in treated mice at 7570 mg/m³ when exposed for 2 hours. Initially all animals exhibited slight inflammations of the eyes and the respiratory tract as well as agitation, followed by a loss of activity. After exposures to values up to 4,000 mg/m³ these symptoms were reversible after the termination of the exposure. Higher concentrations caused further CNS and neuromuscular symptoms (weak reflexes, unsteady gait, respiratory disorders, and tonic-clonic spasms). The dissection revealed injuries in several organs (vascularisation, bleeding, dystrophic changes). Therefore, LC50 was considered to be 7570 mg/m³, when mice were exposed to test chemical via inhalation by vapor for 2 hour exposure.