Thymol

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

weight of evidence

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from publication.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

monograph

Author:

Opdyke et al.

Year:

1982

Bibliographic source:

Food and Chemical Toxicology

Reference 2

Reference Type:

other: authoritative database

Title:

Acute inhalation toxicity study

Author:

IFA GESTIS

Year:

2017

Bibliographic source:

GESTIS SUBSTANCE Database, 2017.

Reference 3

Reference Type:

other: authoritative database

Title:

Acute inhalation toxicity study in rat

Author:

Jay A. Brown

Year:

2017

Bibliographic source:

Haz-Map®

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: As mentioned below

Principles of method if other than guideline:

Acute inhalation toxicity study of test chemical in rat.

GLP compliance:

not specified

Test type:

other: not specified

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Guaiacol

EC Number:

201-964-7

EC Name:

Guaiacol

Cas Number:

90-05-1

Molecular formula:

C7H8O2

IUPAC Name:

2-Methoxyphenol

Details on test material:

Name: 2-MethoxyphenolInChI:1S/C7H8O2/c1-9-7-5-3-2-4-6(7)8/h2-5,8H,1H3Smiles: COc1ccccc1O- Name of test material:Guaiacol- Molecular formula :C7H8O2- Molecular weight :124.1382 g/mol- Substance type:organic- Physical state:Colorless solid

Test animals

Species:

mouse

Strain:

not specified

Sex:

not specified

Details on test animals or test system and environmental conditions:

not specified

Administration / exposure

Route of administration:

inhalation: vapour

Type of inhalation exposure:

not specified

Vehicle:

not specified

Remark on MMAD/GSD:

not specified

Details on inhalation exposure:

not specified

Analytical verification of test atmosphere concentrations:

not specified

Duration of exposure:

2 h

Remarks on duration:

not specified

Concentrations:

Range between 2,000 and 17,000 mg/m³

No. of animals per sex per dose:

not specified

Control animals:

not specified

Details on study design:

- Necropsy of survivors performed: yes- Other examinations performed: Animals were observed for mortality and clinical signs.

Statistics:

not specified

Results and discussion

Preliminary study:

not specified

Effect levels

Sex:

not specified

Dose descriptor:

LC50

Effect level:

7 570 mg/m³ air

Based on:

test mat.

Exp. duration:

2 h

Remarks on result:

other: 50% mortality was observed

Mortality:

50% mortality was observed

Clinical signs:

other: Initially all animals exhibited slight inflammations of the eyes and the respiratory tract as well as agitation, followed by a loss of activity. After exposures to values up to 4,000 mg/m³ these symptoms were reversible after the termination of the exposu

Body weight:

not specified

Gross pathology:

The dissection revealed injuries in several organs (vascularisation, bleeding, dystrophic changes).

Other findings:

not specified

Applicant's summary and conclusion

Interpretation of results:

other: Not classified

Conclusions:

The acute inhalation toxicity dose (LC50) was considered to be 7570 mg/m³, when mice were exposed to test chemical via inhalation by vapor for 2 hour exposure.

Executive summary:

The acute inhalation toxicity study was conducted by using test chemical in mice at the concentration range between 2,000 and 17,000 mg/m³. Animals were observed for mortality and clinical signs. Necropsy was performed. 50% mortality was observed in treated mice at 7570 mg/m³ when exposed for 2 hours. Initially all animals exhibited slight inflammations of the eyes and the respiratory tract as well as agitation, followed by a loss of activity. After exposures to values up to 4,000 mg/m³ these symptoms were reversible after the termination of the exposure. Higher concentrations caused further CNS and neuromuscular symptoms (weak reflexes, unsteady gait, respiratory disorders, and tonic-clonic spasms). The dissection revealed injuries in several organs (vascularisation, bleeding, dystrophic changes). Therefore, LC50 was considered to be 7570 mg/m³, when mice were exposed to test chemical via inhalation by vapor for 2 hour exposure.