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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: no guideline study, few experimental data

Data source

Reference
Reference Type:
publication
Title:
Essential oil of Lippia grata: Pharmacological effects and main constituents
Author:
Viana GSB, Matos FF, Araujo WL, Matos FJA, Craveiro AA
Year:
1981
Bibliographic source:
Quart J Crude Drug Res 19, 1-10

Materials and methods

Principles of method if other than guideline:
Acute toxicity in mice: Thymol was given by i.p. injection to 6 groups of 3-6 adult Swiss mice each (sex at random). The doses ranged from 33.3 to 223 mg/kg body weight for commercial tymol. Mortality and sequential effects during 3 days were determined.
GLP compliance:
no

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
Thymol (no data on purity), purchased from Merck & Co., Germany

Test animals

Species:
mouse
Strain:
not specified
Sex:
not specified

Administration / exposure

Route of administration:
intraperitoneal
Doses:
33.3, 50.0, 73.3, 110.0, 166.6, 233.3 mg/kg bw
No. of animals per sex per dose:
3
Control animals:
not specified

Results and discussion

Effect levels
Sex:
male/female
Dose descriptor:
LD50
Effect level:
ca. 110 other: mg/kg bw
Remarks on result:
other: 3 days LD50

Any other information on results incl. tables

Dose/kg     Death     Sequential Effects

33.3 mg 0/3 no effect
50.0 mg 0/3 slight ataxia
73.3 mg 1/3 ataxia, decreased spontanous motor
activity, death
110.0 mg 3/3 ataxia, decreased spontaneous motor
activity, somnolence, death
166.6 mg 3/3 ataxia, decreased spontaneous motor
activity, somnolence, death
233.3 mg 3/3 ataxia, decreased spontaneous motor
activity, somnolence, death
The animals were observed for a period of 3 days

Applicant's summary and conclusion

Executive summary:

Acute toxicity in mice: Thymol was given by i.p. injection to 6 groups of 3-6 adult Swiss mice each (sex at random). The doses ranged from 33.3 to 223 mg/kg body weight for commercial tymol. Mortality and sequential effects during 3 days were determined.

The approximate 3 days LD50 was 110 mg/kg bw for thymol