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EC number: 201-245-8
CAS number: 80-05-7
Bisphenol A; BPA
Chinese hamster ovary (CHO-WBL) cells were
used for detection of sister chromatid exchanges (SCEs) and chromosome
aberrations (CAs). Bisphenol A was tested in each assay with and without
exogenous metabolic activation. For the SCE trials without metabolic
activation, cells were exposed to Bisphenol A (0, 0.6, 2.4, 8.0, 15, 20,
25, 30, 40, or 50 µg/mL) for approximately 25 hours; for the trails with
metabolic activation, exposure was for two hours. For both testing
conditions, BrdU was added two hours after dosing and Colcemid was added
during the last 2 to 2.5 hours of incubation. Mitotic cells were fixed
on slides, stained with Hoechst 33258, and examined for SCEs by
fluorescence microscopy. Bisphenol A, at 8 µg/mL, produced a small
increase (22%) in the first SCE trial without metabolic activation. Cell
cycle delay was observed at this dose, and this response was not
observed in the second trial, even though higher doses (15 to 30 µg/mL)
were analyzed. The trial with metabolic activation did not induce SCEs
when tested up to toxic levels of BPA, and it was concluded that BPA
does not induce SCE.
In the CA assay without metabolic
activation, cells were exposed to Bisphenol A (0, 20, 30, 40, or 50
ug/mL) for eight hours, then cells were washed and treated with Colcemid
for 2 to 2.5 hours. With metabolic activation, the cells were exposed to
Bisphenol A and the metabolic activation mixture for two hours, washed,
incubated for eight hours, then treated with Colcemid for 2 to 2.5
hours. Cells were harvested, fixed on slides, and stained with Giemsa
for analysis and classification of CAs. In the CA trial without
metabolic activation, Bisphenol A did not induce CAs when tested with
delayed harvest and up to toxic levels. In the first CA trial with
metabolic activation, a positive response was observed at 50 ug/mL, a
dose where cell confluence was reduced by approximately 70%. This
response did not repeat in the second trial, and it was concluded that
Bisphenol A was negative in the CA assay.
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