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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Link to relevant study record(s)

Description of key information

The results of a number of studies are included in this section together with one comprehensive review.  The study chosen for both freshwater and marine invertebrates were conducted in a closed system to reduce active ingredient losses by volatilisation. The best study on fresh water invertebrates for which a robust study is available is that by Abernethy et al 1986. However, the risk assessment conducted by  De Rooij et al 2004 based on the EuroChlor review contains a better study Bobra et al 1984 which achieved a higher classification for reliability than the  study included in the dossier. As this study has a lower LC50 value for freshwater invertebrates it is used as the endpoint. (as a matter of interest the Abernethy 1986 study restates the results from the Bobra 1983 study but calculates the LC50 incorrectly therefore the two studiesare in fact identical). For marine invertebrates the LC50 value is obtained in a similar way from the EuroChlor review. 

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
57.6 mg/L
EC50/LC50 for marine water invertebrates:
7.5 mg/L

Additional information

The results of the risk assessment De Rooij et al 2004 on theEuro Chlor review of studies available on a freshwater and marine invertebrates are shown below.

Acute studies on freshwater invertebrates

Species

Duration

Study type

Endpoint

Conc.

(mg/L)

SF 1000

(ug/L)

Validity

Comments

Reference

Daphnia

magna

48h

S,A,C

LC50

57.6

57.6

1

Bobra et al 1984

48h

S,N,C

LC50

59.6

59.6

2

immobility

Abernethy et al 1986

48h

S,N,C

LC50

>530

>530

2

NOEC 530mg/L

LeBlanc 1980

24h

S,N

EC50

2400

2400

3

immobility

Bringmann & Kuhn 1982

Acute studies on marine invertebrates

Species

Duration

Study type

Endpoint

Conc.

(mg/L)

SF 1000

(ug/L)

Validity

Comments

Reference

Elminius modestus

48h

S,N,C

LC50

7.5

7.5

2

Pearson & McConnel 1975

Artemia salina

24h

S,N

EC50*

24

48

72

113

44

8

113

44

8

2

(3)

(3)

Lack of control data

Sanchez-Fortun et al 1997

Mysidopsis bahia

96h

S,N

LC50

31.2

31.2

4

US EPA 1980

A.salina – three stages tested (24, 48, 72h.

Key:    Analysis A = Yes, N = nominal concentration. Study type S = static, SS = semi-static, FT = flow through. C = closed system.

Rating for validity 1 = valid without restriction, 2 = valid with restrictions, 3 = invalid, 4 = not assignable (inadequate data).

See also Overall Remarks in EuroChlor 1999 endpoint study report.