Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-259-7 | CAS number: 93-58-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Biodegradation in water: screening tests
Administrative data
Link to relevant study record(s)
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)
- Deviations:
- no
- GLP compliance:
- yes
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge (adaptation not specified)
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): laboratory treatment plant processing domestic sewage (Wupperverband)
- Storage conditions: not reported
- Storage length: not reported
- Preparation of inoculum for exposure:
- Pretreatment: none
- Concentration of sludge:
- Initial cell/biomass concentration:
- Water filtered: yes/no - Duration of test (contact time):
- 29 d
- Initial conc.:
- 20 mg/L
- Based on:
- other: Total organic carbon
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Composition of medium: not reported
- Additional substrate: not reported
- Solubilising agent (type and concentration if used): none
- Test temperature: not reported
- pH: not reported
- pH adjusted: no
- Aeration of dilution water: no
- Suspended solids concentration: 25 mg dry matter/L
- Continuous darkness: not reported
TEST SYSTEM
- Culturing apparatus:
- Number of culture flasks/concentration: 1
- Method used to create aerobic conditions: aeration with CO2-free air
- Measuring equipment: not reported
- Test performed in closed vessels due to significant volatility of test substance:
- Test performed in open system:
- Details of trap for CO2: bottles filled with barium hydroxide solution or caustic soda
SAMPLING
- Sampling method: continuous air flow through CO2 trap
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Toxicity control: no - Reference substance:
- aniline
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 10
- Sampling time:
- 2 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 63
- Sampling time:
- 13 d
- Parameter:
- % degradation (CO2 evolution)
- Value:
- 62
- Sampling time:
- 29 d
- Details on results:
- The 10-day window was fulfilled.
- Results with reference substance:
- The reference substance aniline achieved a degradation of 67% within 29 days demonstrating the suitability of the sewage sludge.
- Validity criteria fulfilled:
- yes
- Interpretation of results:
- readily biodegradable
- Conclusions:
- The substance is readily biodegradable.
- Executive summary:
The ready biodegradability of the substance methyl benzoate was studied under GLP conditions in a carbon dioxide evolution test according to EU Method C.4-C over a period of 29 days. A defined volume of nutrient medium was inoculated with activated sludge from a treatment plant processing domestic sewage at a concentration of 25 mg dry matter/L. The test substance was added to this mixture at a concentration of 20 mg C/L corresponding to 28 mg/L substance. The bottles were aerated with CO2-free air. The biodegradation of the test substance was studied by determining the amount of CO2 generated during a testing period of 28 days. To this end, the air stream was directed through barium hydroxide solutions/caustic soda and the amount of CO2 was determined by titration of remaining hydroxide/caustic soda. The generated CO2 was related to the theoretical amount of CO2 released in case of complete oxidation of the test substance to obtain the fraction of substance that was biodegraded. Evolution of CO2 was surveyed over a period of 29 days. A fraction of 62% of the substance had been degraded at the end of the observation period. The 10-day window was fulfilled. A parallel degradation test was conducted with aniline, which resulted in a fraction of 67% of the reference substance being biodegraded showing the suitability of the activated sludge. In conclusion, the substance was considered as readily biodegradable according to the OECD criteria.
Reference
Description of key information
The substance was readily biodegradable (fulfilling the 10-day window) in a standard CO2 evolution study over a period of 28 days.
Key value for chemical safety assessment
- Biodegradation in water:
- readily biodegradable
- Type of water:
- freshwater
Additional information
The biodegradability of methyl benzoate was studied in a CO2 evolution test in accordance with EU Method C.4-C over a testing period of 29 days. The test was valid according to the criteria given in the testing guideline. A degradation of 62% of the test substance was found at the end of the testing period (degradation of the reference substance aniline was 69%). The 10-day window was fulfilled.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
