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Valid acute aquatic toxicity studies in three freshwater species from different trophic levels are available for the substance methyl benzoate. According to the test results, fish and aquatic invertebrates are more sensitive to acute exposure to the substance than algae, with fish being the most sensitive species. In the absence of long-term toxicity data and following the rules of PNEC derivation, the lowest L(E)C50 value from the available acute aquatic toxicity studies will be used further in the hazard assessment, i.e. the 96-hour LC50 value in fish of 23 mg/L. Methyl benzoate exhibits a low inhibitory effect on microorganisms of activated sludge with an EC50 value of 815 mg/L over a period of 3 hours.