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EC number: 202-259-7 | CAS number: 93-58-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Dermal absorption
Administrative data
- Endpoint:
- dermal absorption in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Study was performed according to scientific principles.
Data source
Reference
- Reference Type:
- publication
- Title:
- An experimentally based approach for predicting skin permeability of chemicals and drugs using a membrane-coated fiber array
- Author:
- Xia X-R, Baynes RE, Monteiro-Riviere NA, Riviere JE
- Year:
- 2 007
- Bibliographic source:
- Toxicology and Applied Pharmacology 221: 320-8
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- With a membrane-coated fiber (MCF) technique relative strengths of the molecular interactions (the partition coefficients) were investigated. A multiple MCF polymer membranes consisted of polydimethylsiloxane (PDMS) for lipophilic, CarboWax (Wax) for hydrogen bonding and polyacrylate (PA) for π*-electron interactions. With porcine skin, experimental skin permeability log (kp) was determined, which were compared with estimated skin permeability from an empirical approach.
- GLP compliance:
- no
Test material
- Reference substance name:
- Methyl benzoate
- EC Number:
- 202-259-7
- EC Name:
- Methyl benzoate
- Cas Number:
- 93-58-3
- Molecular formula:
- C8H8O2
- IUPAC Name:
- methyl benzoate
Constituent 1
- Radiolabelling:
- no
Test animals
- Species:
- pig
- Strain:
- other: Yorkshire
- Sex:
- female
Administration / exposure
- Control animals:
- no
- Details on in vitro test system (if applicable):
- SKIN PREPARATION
- Source of skin: porcine skin of weaning
- Type of skin: dorsal area
- Preparative technique: dermatomed
- Thickness of skin (in mm): 0.35
- Membrane integrity check: no data
- Storage conditions: no data
- Justification of species, anatomical site and preparative technique: no data
PRINCIPLES OF ASSAY
- Diffusion cell: two-compartment Teflon flow-through diffusion cell
- Receptor fluid: Krebs-Ringer bicarbonate buffer spiked with dextrose and bovine serum albumin (4.5 %)
- Solubility of test substance in receptor fluid: no data
- Static system: Brinkman circulator
- Flow-through system: 4.0 mL/h
- Test temperature: 37 °C
- Humidity: no data
- Occlusion: no data
- Reference substance(s): no data
- Other: pH was maintained between 7.3 and 7.5
Results and discussion
Percutaneous absorption
- Remarks on result:
- other: Under experimental conditions in vitro with porcine skin, methyl benzoate was metabolised. The skin permeability log(kp) was estimated from refined Potts and Guy models in USEPA Supplemental Guidance for Dermal Risk Assessment and was -2.16 cm/h.
Any other information on results incl. tables
Three sets of partition coefficients (logK) were measured with three MCFs (PDMS, PA and Wax) for Methyl benzoate and were 1.882, 2.015 and 1.561, respectively.
In the in vitro experiment with porcine skin, the receptor solution was formulated to mimic the microvascular circulation of the skin, composed of 4.5 % bovine serum albumin in a Krebs-Ringer bicarbonate buffer with dextrose (0.12 %). Since Methyl benzoate was metabolized under the experimental conditions, the skin permeability value log(kp) was not obtained. However, skin permeability log(kp) was estimated from refined Potts and Guy models in USEPA Supplemental Guidance for Dermal Risk Assessment and was -2.16 cm/h.
Applicant's summary and conclusion
- Conclusions:
- Methyl benzoate was metabolized under the experimental conditions in vitro, therefore the skin permeability value log(kp) was not obtained. The skin permeability log(kp) was estimated by empirical approach from refined Potts and Guy models in USEPA Supplemental Guidance for Dermal Risk Assessment and was -2.16 cm/h.
- Executive summary:
The MCF array approach is used to stimulate the biological processes, where passive diffusion is the rate limiting transport mechanism. The partition coefficients were determined for Methyl benzoate by multiple MCF membranes. For example, logK through polydimethylsiloxane (PDMS), where lypophilic interactions were measured was 1.882, logK through polyacrylate (PA), where π*-electron interactions were measured was 2.015 and logK through CarboWax (Wax), where hydrogen bonding were measured was 1.561.
Under experimental conditions in vitro with porcine skin, methyl benzoate was metabolised, therefore the skin permeability coefficient log (kp) was not determined. Using the empirical approach from refined Potts and Guy models in USEPA Supplemental Guidance for Dermal Risk Assessment, the log(kp) was calculated to be -2.16 cm/h.
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