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EC number: 202-259-7 | CAS number: 93-58-3
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- Ecotoxicological Summary
- Aquatic toxicity
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1992-06-15 to 1992-06-17
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Details on sampling:
- not applicable
- Vehicle:
- no
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Source: Activated sludge from a laboratory wastewater treatment plant
- Preparation of inoculum for exposure: not reported
- Pretreatment: no pretreatment
- Initial biomass concentration: 6 g dry matter/L - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- not applicable
- Hardness:
- not reported
- Test temperature:
- not reported
- pH:
- not reported
- Dissolved oxygen:
- not reported
- Salinity:
- not applicable
- Nominal and measured concentrations:
- Nominal: 560, 1000, 1800, 3200, 5600 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel: not reported
- Type (delete if not applicable): not reported
- Material, size, headspace, fill volume: not reported
- Aeration: not reported
- No. of vessels per concentration (replicates): not reported
- No. of vessels per control (replicates): not reported
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: not reported
- Total organic carbon: not reported
- Particulate matter: not reported
- Metals: not reported
- Pesticides: not reported
- Chlorine: not reported
- Alkalinity: not reported
- Ca/mg ratio: not reported
- Conductivity: not reported
- Culture medium different from test medium: not reported
OTHER TEST CONDITIONS
- Adjustment of pH: not reported
- Photoperiod: not reported
- Light intensity: not reported
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : respiration rate of sewage sludge - Reference substance (positive control):
- yes
- Remarks:
- 3,5-dichlorophenol
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 815 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
- Details on results:
- The inhibition of the respiration of the sludge was between 30% at the lowest test concentration of 560 mg/L and 92.3% at the highest test concentration of 5600 mg/L
- Results with reference substance (positive control):
- The study results with the reference substance are not reported.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The substance inhibited the microbial activity of sewage sludge and the EC50 value was 815 mg/L after 3 hours.
- Executive summary:
The toxicity of the substance methyl benzoate to bacteria in sewage sludge was tested under GLP conditions in a static system in accordance with OECD guideline No 209. The respiration rate of activated sludge from a laboratory wastewater treatment plant was determined at a sludge concentration of 6 g dry matter/L in the absence of test substance. Then the corresponding respiration rate of the sludge exposed to nominal test concentrations of 560, 1000, 1800, 3200 and 5600 mg/L for 3 hours was determined and compared to the respiration rate of untreated sludge. The microbial activity of the sludge was inhibited at all test concentrations and reduced by 30% to 92.3%. The EC50 value for inhibition was 815 mg/L after 3 hours. The sensitivity of the activated sludge was tested and confirmed with the reference substance 3,5 -dichlorophenole.
Reference
Description of key information
The 3-hour EC50 value for inhibition of respiration of activated sludge was 815 mg/L.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 815 mg/L
Additional information
The inhibition of respiration of activated sludge following three hours of exposure to methyl benzoate at a series of test concentrations was determined in a valid study in accordance with OECD TG 209. Activated sludge was taken from a laboratory sewage treatment plant according to OECD. The EC50 value for inhibition of the respiration rate of activated sludge was 815 mg/L after 3 hours of exposure.
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