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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

 The substance was moderately toxic in a 96-hour test with zebra fish resulting in a LC50 value of 23 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
23 mg/L

Additional information

A valid study on the acute toxicity of methyl benzoate to freshwater fish according to EU Method C.1 (1992) is available, in which zebra fish were exposed to the substance under semi-static conditions for a period of 96 hours. The LC50 found in the study was 23 mg/L. Information on a prolonged acute toxicity test with guppy was published (Verhaar et al. 1999). Details on the actual study were not provided in this publication. The 96-hour LC50 was 40 mg/L and the 14-day LC50 was 28.6 mg/L, which is quite similar to the value found in the study with zebra fish. Another acute toxicity study in three freshwater species - trout, bluegill sunfish and goldfish - resulted in mortality of trout and bluegill fish at a nominal test concentration of 5 ppm. However, this test cannot be considered as valid mainly due to the small number of test animals.

It can be concluded that fish appears to be the most sensitive aquatic species with regard to acute exposure to methyl benzoate when compared to other aquatic organisms.