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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Additional toxicological data

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Administrative data

Endpoint:
additional toxicological information
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: sufficient documented for evaluation

Data source

Reference
Reference Type:
publication
Title:
Ueber Methaemoglobinaemie unter Mentholeinwirkung sowie erste Versuche mit dem Impedance-Pneumographen über die Beeinflussung der Atmung
Author:
Berger H, Hohenwallner W, Arakelian N
Year:
1967
Bibliographic source:
in Menthol and Menthol-containing external remedies, ed. Dost, F.H. and Leiber, B., page 120-134, Georg Thieme Verlag Stuttgart (1967)

Materials and methods

Type of study / information:
methaemoglobin bilding in vitro
Principles of method if other than guideline:
haemolysates of infantile erythrocytes were mixed with 50, 100, 200 and 500 gamma menthol
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Menthol
EC Number:
201-939-0
EC Name:
Menthol
Cas Number:
89-78-1
Molecular formula:
C10H20O
IUPAC Name:
2-isopropyl-5-methylcyclohexanol

Results and discussion

Any other information on results incl. tables

When haemolysates of infantile erythrocytes were mixed with 50, 100, 200 and 500 gamma menthol, the methaemoglobin content rose by up to 100 %, namely from 0.7% to 1.5%; these values values are absolutely within physiological limits.

Applicant's summary and conclusion

Conclusions:
When haemolysates of infantile erythrocytes were mixed with 50, 100, 200 and 500 gamma menthol, the methaemoglobin content rose by up to 100 %, namely from 0.7% to 1.5%; these values values are absolutely within physiological limits.
Executive summary:

in vitro test: 

when haemolysates of infantile erythrocytes were mixed with 50, 100, 200 and 500 gamma menthol, the
methaemoglobin content rose by up to 100 %, namely from 0.7 to 1.5%; these values are absolutely within physiological limits and this effect could be neutralized with vitamin C (test substance: unspecified isomer)