Registration Dossier

Ecotoxicological information

Short-term toxicity to fish

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1990-03-19 till 1990-04-17
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to
Guideline:
other: UBA-Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, LC0, LC50, LC100; 48-96 h" (Mai 1984)
Version / remarks:
Guideline proposal of the German Federal Environmental Agency (UBA)
GLP compliance:
yes
Specific details on test material used for the study:
SOURCE OF TEST MATERIAL
- Source and lot/batch No.of test material: 131136
Analytical monitoring:
yes
Details on sampling:
direct weighted
Vehicle:
no
Details on test solutions:
test medium (synthetic origin, prepared according to ISO)
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
- origin: West-Aquarium (Bad Lauterberg, Germany)
- ventilated test system
- loading rate: 10 animals/vessel (3 month old)
- Length: 2.5 to 3.5 cm
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Test temperature:
22.3°C
pH:
7.6
Dissolved oxygen:
8.5 mg/L
99.6 %
Nominal and measured concentrations:
Nominal: 7.8, 11, 16, 22 and 31 mg/L
Measured (in mean): 6.4, 9.3, 11.3, 18.7 and 26.2 mg/L
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
16 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
22.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
31 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The fish were exposed to 5 nominal test substance concentrations in the range of 7.8 to 31 mg/L. In all test media, undissolved substance particles
remained on the water surface. Analytical measurements revealed that the test substance concentrations decreased below 80 % of the nominal within 96 h, probably due to volatilization.
Validity criteria fulfilled:
yes
Remarks:
- dissolved oxygen concentration remained above 60% of the air-saturation value throughout the exposure period. - No mortality in the controls by the end of the test.
Conclusions:
In a study with fish (Danio rerio) a LC50 of 22.3 mg/L (geom. mean from nominal concentration) after 96 hours is obtained.
Executive summary:

The study was conducted according to Umweltbundesamt (UBA) -Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, Mai 1984" with fish (Danio rerio). The fish were exposed to 5 nominal concentrations of DL-Menthol in the range of 7.8 to 31 mg/L ( 7.8, 11, 16, 22 and 31 mg/L). 10 fish were tested per concentration level. A LC50 of 22.3 mg/L (geom. mean from nominal concentration) after 96 hours is obtained.

Description of key information

The study was conducted according to Umweltbundesamt (UBA) -Verfahrensvorschlag: "Letale Wirkung beim Zebrabaerbling Brachydanio rerio, Mai 1984" with fish (Danio rerio). The fishs were exposed to 5 nominal concentrations of DL-Menthol in the range of 7.8 to 31 mg/L ( 7.8, 11, 16, 22 and 31 mg/L). 10 fish were tested per concentration level. A LC50 of 22.3 mg/L (geom. mean from nominal concentration) after 96 hours is obtained.

Key value for chemical safety assessment

LC50 for freshwater fish:
22.3 mg/L

Additional information