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EC number: 201-939-0 | CAS number: 89-78-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
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- Boiling point
- Density
- Particle size distribution (Granulometry)
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- Auto flammability
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- Oxidation reduction potential
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- Additional physico-chemical information
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- Endpoint summary
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- Environmental data
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- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
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- Toxicological Summary
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Toxicity to microorganisms
Administrative data
Link to relevant study record(s)
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 12/1989
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- guideline study with acceptable restrictions
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
- Deviations:
- no
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- ISO 8192 (Water quality - Test for inhibition of oxygen consumption by activated sludge for carbonaceous and ammonium oxidation)
- Deviations:
- no
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Test organisms (species):
- activated sludge
- Details on inoculum:
- - Initial biomass concentration: 6.0 g/L (dry weight)
- storage of the sludge: aeration of the activated sludge at 20°C ± 2°C, daily fed with synthetic medium
- laboratory scale sewage treatment unit (3L) treating predominantly industrial sewage. (Location: Bürrig / Germany)
- first, the sludge was settled and the supernatant was decanted
- after centrifuging (20 min at 4000 rpm and 20°C) the supernatant was decanted
- approx. 1 g of the wet sludge was dried in order to calculate the amount of sludge to achieve a concentration of activated sludge of 6 g/l (dry weight) suspended solids
- the calculated amount of sludge was first dissolved in synthetic medium and than filled up to a defined end volume with deionised water - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 3 h
- Test temperature:
- 19.8 °C
- pH:
- 8.0 - 8.3
- Dissolved oxygen:
- O2 - Start: 5.2 - 8.4 mg
O2 - End: 3.0 - 8.2 mg - Nominal and measured concentrations:
- nominal concentrations: (preliminary Test) 100, 1000 and 10000 mg/L
nominal concentrations: (primary test) 100, 180, 320, 560 and 1000 mg/L - Details on test conditions:
- Type: aquatic
- Reference substance (positive control):
- yes
- Remarks:
- 3,5-Dichlorophenol
- Key result
- Duration:
- 3 h
- Dose descriptor:
- EC50
- Effect conc.:
- 306 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Duration:
- 3 h
- Dose descriptor:
- EC10
- Effect conc.:
- 117 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- inhibition of total respiration
- Details on results:
- The concentrations of the test item and reference compound were not confirmed by analytical methods, but instead based on nominal concentrations.
The respiration rate for each concentration was determined graphically from the linear part of the curve of O2-content versus time. The inhibitory effect of the test item at a particular concentration is expressed as a percentage of the mean of the respiration rates of two controls. An EC50 value was calculated, using Probit – analyses, from the inhibition of the respiration rates at different test item concentrations. - Results with reference substance (positive control):
- EC50 of the reference compound 3,5-Dichlorophenol is < 20 mg/L
- Validity criteria fulfilled:
- yes
- Remarks:
- on the basic of graphic evaluation: the EC50 for 3,5-DCP was 20 mg/L and between the prescribed limits of 5 and 30 mg/L.
- Conclusions:
- For DL-Menthol an EC50 of 306 mg/L was observed.
- Executive summary:
A study with activated sludge with a duration of 3 hours was performed according to the OECD Guideline 209. The sludge was exposed to five different concentrations (nominal) of DL-Menthol: 100, 180, 320, 560 and 1000 mg/L. The respiration rate of each mixture was determined after aeration periods of 3 h. An EC10 of 117 mg/L and an EC50 of 306 mg/L was determined by probit analysis.
Reference
Results:
- preliminary Test:
Test conc. [mg/L] | Respiratory rate [mg/L*h] | Inhibition [%] | Reference substance [mg/L] | Respiratory rate [mg/L*h] | Inhibition [%] |
100 | 22 | 0 | 1.0 | 22 | 0 |
1000 | 3 | 85 | 20 | 5 | 75 |
10000 | 0 | 100 |
Results:
- primary Test:
Test conc. [mg/L] | Respiratory rate [mg/L*h] | Inhibition [%] | Reference substance [mg/L] | Respiratory rate [mg/L*h] | Inhibition [%] |
100 | 19 | 7 | 1.0 | 22 | 0 |
180 | 17 | 19 | 20 | 5 | 75 |
320 | 7 | 67 | |||
560 | 4 | 80 | |||
1000 | 2 | 88 |
Description of key information
A study with activated sludge with a duration of 3 hours was performed according to the OECD Guideline 209. The sludge was exposed to five different concentrations (nominal) of DL-Menthol: 100, 180, 320, 560 and 1000 mg/L. The respiration rate of each mixture was determined after aeration periods of 3 h. An EC10 of 117 mg/L and an EC50 of 306 mg/L was determined by probit analysis.
Key value for chemical safety assessment
- EC50 for microorganisms:
- 306 mg/L
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