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EC number: 239-622-4
CAS number: 15571-58-1
DOT(EHMA) induced no visible effects in zebra-fish (Brachydanio rerio) at or below a Water Accommodated Fraction (WAF) prepared at a loading rate of 100 mg/L over 96 hours. Based on average measured concentrations the NOEC corresponded to 24.8 mg DOT(EHMA) per litre (3.91 mg tin per litre).
The Migchielsen, 2004, study (as amended by Migchielsen and de Haan,
2005) was chosen as the key study for this endpoint. The study was a
96-hour Acute toxicity study in zebra-fish with DOT(EHMA), conducted
according to the EEC directive 92/69, Part C. L "Acute toxicity for
fish"; and the OECD guideline No. 203: "Fish Acute Toxicity Test",
Adopted 17 July, 1992.
The batch of DOT(EHMA) tested was a white to light yellow liquid
enriched with 87.5% DOT(EHMA). DOT(EHMA) was not soluble in test medium
at the concentrations tested. Preparation started with a supersaturated
dispersion at 100 mg/l, which was stirred for approximately 24 hours
applying an ultra thurrax. The dispersion was then left to stabilise for
one hour. Subsequently, the Water Accommodated Fraction (WAF) was
separated from the undissolved fraction of test substance by means of
siphoning. The lower test concentrations were prepared by subsequent
dilution of the WAF in test medium. All test solutions were renewed
daily and all test vessels were pre-conditioned for 30 minutes with the
respective solution. A limit test was combined with a range-finding test
in a semi-static system with daily renewal. Seven zebra-fish per test
group were exposed to a blank-control and a WAF prepared at a DOT(EHMA)
loading rate of 100 mg/l in the limit test, while three zebra-fish per
test group were exposed to dilutions containing 0.1, 1.0 and 10% of the
WAF in the additional range-finding test. Samples for analytical
confirmation of actual exposure concentrations were taken from freshly
prepared solutions of the blank-control and the undiluted WAF at t=0 h
and t=72 h and from the 24-hour old solutions at t=24 h and t=96 h.
Analysis showed that the measured tin concentrations decreased slightly
during the 24-hour renewal periods. Analyses further showed poor
repeatability of test solution preparation. This was not unexpected
considering the extremely low solubility of the test substance in the
test medium in combination with the difficulties encountered with the
separation of the undissolved from the dissolved fraction, which is a
common feature when testing WAFs. The study met the acceptability
criteria prescribed by the protocol and the guidelines and was
considered valid. DOT(EHMA) induced no visible effects in zebra-fish
(Brachydanio rerio) at or below a Water Accommodated Fraction (WAF)
prepared at a loading rate of 100 mg/l. Based on average measured
concentrations the NOEC corresponded to 24.8 mg DOT(EHMA) per litre
(3.91 mg tin per litre).
The Grade, 1993, study is presented as supporting information only, the
study was conducted according to EC Method C.1 and the result presented
was a 96-hour LC50 of >5.8 mg/L.
two reports indicate that DOTE is not toxic or harmful to fish up to its
expected water solubility (< 1.0 mg/L).
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