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EC number: 204-616-2
CAS number: 123-30-8
The observed endpoints effects of the test item on the growth of the
freshwater green algal species Pseudokirchneriella subcapitata are
summarized as: ErC10=0.083, ErC50=0.25, NOEC 0.017, LOEC 0.031 mg/L. The
biological results were related to the geometric mean of the
The impact of the test item on the growth of the freshwater green algal
species Pseudokirchneriella subcapitata was investigated in a 72-hour
static test according to the OECD Guideline 201 (2006) and the
Commission Regulation (EU) No 2016/266, C.3, and following the GLP.
The nominal concentrations of the test item of 0.030, 0.090, 0.27, 0.81,
2.4, and 7.3 mg/L were tested in parallel with a control (test water
without test item). A stock solution of the nominal concentration of 73
mg/L was prepared by dissolving 73.03 mg of the test item completely in
1000 mL of test water. This stock solution was serially diluted with
test water to prepare the test media of the different test
concentrations as stated above. For measurement of the actual
concentrations of the test item, duplicate samples were taken from the
test media of all test concentrations at the start and at the end of the
test. As the test item is highly instable, additional stability samples
were taken after 24 and 48 hours following the recommendation of the
At the start of the test, the measured test item concentrations in the
test media with the concentrations of 0.030 to 7.3 mg/L in the range of
81 to 100% of the nominal values). The results show the correct
preparation of the test media. After 24 hours test period, the test item
concentrations had strongly decreased. The measured concentrations after
24, 48 and 72 hours were 0.012, 0.017, 0.031, 0.15, 0.39 and 0.96 mg/L.
The mean measured test item concentrations were calculated as the
geometric mean of the concentrations measured at all sampling dates (0,
24, 48, and 72 hours). All the validity criteria were fulfilled
according to the OECD guideline 201. The observed endpoints effects of
the test item on the growth of the freshwater green algal species
Pseudokirchneriella subcapitata are summarized as: ErC10=0.083,
ErC50=0.25, NOEC 0.017, LOEC 0.031 mg/L. The biological results were
related to the geometric mean of the concentrations measured.
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