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Toxicological information

Acute Toxicity: other routes

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Administrative data

Endpoint:
acute toxicity: other routes
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Study well documented, meets generally accepted scientific principles, acceptable for assessment. Reliability assessment as per that quoted in IUCLID 4 (2000) data review

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
The toxicity of tetraethyl lead and related alkyl metallic compounds
Author:
Cremer, J.E.
Year:
1961
Bibliographic source:
Annals of Occupational Hygiene, Vol. 3, pp 226-230
Reference Type:
publication
Title:
Biochemical studies on the toxicity of tetraethyl lead and othe organo-lead compounds
Author:
Cremer, J.E.
Year:
1959
Bibliographic source:
British Journal of Industrial Medicine, Vol 16, pp191-199

Materials and methods

Principles of method if other than guideline:
A comparison of the action of organolead compounds was made under in vivo and in vitro conditions. LD50 values were determined. Biochemical studies were also used to understand the mechanism of TEL poisoning. Thin slices of rat cerebral cortex were incubated and the rate of oxygen consumption measured. After 1 hour, the levels of lactic acid and pyruvic acid were determined. Othe tissue samples were analysed for TEL and triethyllead content
GLP compliance:
not specified

Test material

Reference
Name:
Unnamed
Type:
Constituent

Test animals

Species:
rat
Strain:
not specified
Sex:
male
Details on test animals and environmental conditions:
175-230 g body weight maintained on a MRC diet

Administration / exposure

Route of administration:
intravenous
Vehicle:
ethanol
Details on exposure:
Injected once into tail vein total injection (<0.1ml)
Doses:
Each rat received no more than 0.1 ml of 40 mg TEL/ml stock solution. Dose: 20 or 15.4 mg / kg bw

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LD50
Effect level:
15.4 mg/kg bw
Based on:
test mat.
Sex:
male
Dose descriptor:
LC50
Effect level:
13.2 - 18 mg/kg bw
Based on:
test mat.
Sex:
male
Dose descriptor:
LD100
Effect level:
20 mg/kg bw
Based on:
test mat.
Mortality:
All rats died at 20 mg/kg

Any other information on results incl. tables

Estimation of triethyl lead (ug/g wet wt) in tissues of rats injected with 20 mg/kg of TEL

 Time after injection  Blood  Liver  Kidney  Brain
 4 hours  76  45  23  2.4
 24 hours  62  29  25  19

Applicant's summary and conclusion

Conclusions:
The LD50 value was 15.4 mg/kg for TEL. A value of 11.2 mg/kg for the triethyl lead product was also reported.
Executive summary:

Within 24 - 48 hours of injection the rats became very excitable. This was followed by the onset of generalised tremors which became increasingly severe often accompanied by intermittent convulsions leading to death. Rats which did not develop severe tremors recovered within a few days and returned to normal behaviour. The LD50 value was 15.4 mg/kg for TEL. A value of 11.2 mg/kg for the triethyl lead product was also reported.

Brain slices from rats killed 4 hours after being injected with 20 mg/kg TEL showed lower oxygen consumption and increased lactic acid content. The altered metabolic picture was identical to rats given triethyl lead. It is proposed this confirms TEL is converted to triethyl lead in the body.