Registration Dossier
Registration Dossier
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 202-436-9 | CAS number: 95-63-6
Dermal absorption
Administrative data
- Endpoint:
- dermal absorption in vitro / ex vivo
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Non-GLP, non-guideline in-vitro experimental study, published in peer reviewed literature, minor restrictions in reporting but otherwise adequate for assessment.
Data source
Reference
- Reference Type:
- publication
- Title:
- In vitro evaluation of the efficacy of skin barrier creams and protective gloves on percutaneous absorption of industrial solvents
- Author:
- Korinth, G., Geh, S., Schaller, K.H. and Drexler, H
- Year:
- 2 003
- Bibliographic source:
- Int. Arch. Environ. Health, 76, 382-386
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- In an in vitro skin permeability study, the permeation of 1,2,4-trimethylbenzene through human skin was determined using static Franz diffusion cells.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- 1,2,4-trimethylbenzene
- EC Number:
- 202-436-9
- EC Name:
- 1,2,4-trimethylbenzene
- Cas Number:
- 95-63-6
- Molecular formula:
- C9H12
- IUPAC Name:
- 1,2,4-trimethylbenzene
- Details on test material:
- - Name of test material (as cited in study report): 1,2,4-trimethylbenzene
- Purity: >99%
- Source; Fluka (Germany)
Constituent 1
- Radiolabelling:
- no
Test animals
- Species:
- human
- Sex:
- not specified
- Details on test animals or test system and environmental conditions:
- Human skin from one donor obtained from a local hospital and stored for approximately 1 week at -20C.
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- other: neat TMB, 10 or 50% TMB in ethanol
- Duration of exposure:
- 8h
- Doses:
- 128 µL/0.64cm3
Results and discussion
- Absorption in different matrices:
- Steady state status had not been attained after 8 h exposure.
64 µg penetrated untreated skin from neat 1,2,4-TMB, 77 µg penetrated from a 50% ethanolic solution of 1,2,4-TMB.
The presence of skin barrier cream enhanced penetration of 1,2,4-TMB from ethanolic solutions.
1,2,4-TMB was detected in the receptor fluid within 30 min of application to glove material.
Percutaneous absorption
- Dose:
- 128 µl/0.64cm2
- Parameter:
- percentage
- Absorption:
- ca. 0.1 %
- Remarks on result:
- other: 8 h
- Remarks:
- value calculated by reviewer = 0.057% (not reported in original publication)
Applicant's summary and conclusion
- Executive summary:
Steady state status had not been attained after 8 h exposure.
64 µg (0.057%) penetrated human skin when 1,2,4-TMB was applied at a rate of 128 µL/0.64 cm2.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
