Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 216-823-5
CAS number: 1675-54-3
The objective of this study was to evaluate the test article, Bisphenol A-bis-(3-chlor-2-hydroxypropyl)-ether
(BADGE-2HCL), for the ability to induce reverse mutations either in the
presence or absence of mammalian microsomal enzymes at 1) the histidine
locus in the genome of several strains of Salmonella typhimurium and at
2) the tryptophan locus of Escherichia coli strain WP2«vrA. This assay
satisfied the following guidelines: U.S. EPA (1998), EEC (2000), and
The concentrations tested in the mutagenicity assay were selected based
on the results of a , rangefinding assay using tester strains TA 100 and
WP2wvrA and ten concentrations of test article ranging from 6.67 to 5000
u,g per plate, one plate per concentration, both in the presence and absence
of S9 mix.
The tester strains used in the mutagenicity assay were Salmonella
typhimurium tester strains TA98, TA100, TA1535, and TA1537 and
Escherichia coli tester strain WP2uvrA. The assay was conducted with a
minimum of six concentration levels of test article in both the presence
and absence of S9 mix along with concurrent vehicle and positive
controls using three plates per concentration. The concentrations tested
in the mutagenicity assay with the Salmonella tester strains ranged from
1.00 to 1000 \ig per plate in both the presence and absence of S9 mix
(0.333 to 1000 ug per plate with TA 1537 in the absence of S9 mix). The
concentrations tested in the
mutagenicity assay with Escherichia coli tester strain WP2uvrA ranged
from 10.0 to 5000 ug per plate in both the presence and absence of S9
mix. The results of the initial mutagenicity assay were confirmed in an
The results of the Salmonella-Escherichia co/i/Mammalian-Microsome
Reverse Mutation Assay Preincubation Method with a Confirmatory Assay
indicate that under the conditions of this study, the test article,
BADGE-2HCL, did not cause a positive increase in the mean number of revertants
per plate with any of the tester strains either in the presence or
absence of microsomal enzymes prepared from Aroclor™-induced rat liver
(S9). Hence, BADGE-2HCL was considered to be non mutagenic in this assay.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
Welcome to the ECHA website. This site is not fully supported in Internet Explorer 7 (and earlier versions). Please upgrade your Internet Explorer to a newer version.
Do not show this message again