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EC number: 216-823-5
CAS number: 1675-54-3
Based on the chemical oxygen demand for the test material, the
test material attained 5% degradation after 28 days and therefore cannot
be considered to be readily biodegradable under the strict terms and
conditions of OECD Guideline No 301F.
The compound specific analyses conducted
on Day 0 using gas chromatography analysis showed the measured
concentrations in the test material plus inoculum vessels to range from
87% to 90% of the nominal value. In
the abiotic test material vessels the measured concentrations ranged
from 92% to 99% of the nominal value. Examination
of the chromatograms from Day 0 of the test period showed one peak
attributable to the test material in all vessels containing the test
The compound specific analyses conducted
on Day 28 showed measured concentrations in the test material plus
inoculum vessels to range from 15% to 16% of nominal values. Two
new peaks in the chromatogram were identified as the mono-diol and
di-diol of the test material using liquid chromatography mass
the abiotic vessels the measured concentration of the test material was
9% of the nominal value with two additional peaks which were identified
as the mono-diol and di-diol of the test material using liquid
chromatography mass spectrometry.
Although the results from chemical
analysis on Day 28 indicated that there was an approximate 85% loss of
test material from the inoculated test material vessels, this loss was
considered not to be due to biological degradation as a similar loss was
observed in the abiotic vessels. The
two additional peaks shown in the chromatograms of the test material and
abiotic control vessels analysed using liquid chromatography mass
spectrometry on Day 28 confirmed that hydrolysis of the test material
occurred over the test period.
The oxygen consumption values from the
inoculated test material vessels confirmed no biological degradation of
the test material occurred over the test period.
Table 1: BOD and
Mean Biodegradation (%)
R2= Replicates 1 and 2
The material has been tested for biodegradation in an enhanced ready
test following the OECD 301F (oxygen depletion) design. Enhancement
consisted of coating the material to silica gel to address the low
solubility and the high viscosity of the test material. For a series of
similar materials (epoxydes), respiration rates in test vessels were
significantly lower than in the controls. This is taken as a sign of
inhibition of the inoculum that is more relevant than the toxicity
control. Based on these findings, test material concentration was
reduced to 20 mg/l. After 28 days of exposure, 5% of the test material
has mineralised. Test material specific analysis revealed a substantial
elimination of the diepoxide with the mono- and di-diol as relevant
products formed. At termination of the study (day 28) 18% of the initial
measured concentration could be recovered. The same elimination was
observed in the inhibited reactions mixtures (sodium azide) indicating
that this is not due to a biological process (Harlan, 2010). The pseudo
first order rate of elimination in activated sludge at 20 °C is - 0.0565
Not readily biodegradable. Significant hydrolysis to form mono- and di-diol from the di-epoxides at a rate of -0.0565 [1/d] at 20°C.
Testing according to the modified Sturm test (OECD 301B) at 10 and 20 mg
DOC per Litre (approx 13 and 27 mg test material/L) indicated only
limited mineralisation of 6 and 12%, respectively, which is attributed
to the low solubility of the test material (Ciba-Geigy Limited 1985).
Likewise, BOD testing at 10 mg/L ThOD (approx 4 mg/L test material)
showed no decrease in Oxygen at day 5, 10 and 20 (TDCC, 1975).
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