Registration Dossier

Administrative data

Endpoint:
toxicity to soil microorganisms
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Jan 14 - June 9, 2008
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: study performed according to international guidelines and GLPs using the commercial product as test article and published in the literature.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
2008
Reference Type:
publication
Title:
Terrestrial toxicity evaluation of decabromodiphenyl ethane on organisms from three trophic levels.
Author:
Hardy et al.
Year:
2011
Bibliographic source:
Ecotoxicol Environ Saf 74(4):703-10.
Reference Type:
publication
Title:
Results of terrestrial and aquatic studies on the brominated flame retardant, decabromodiphenyl ethane, a.k.a., Ethane 1,2 bis(pentabromophenyl)
Author:
Hardy et al.
Year:
2009
Bibliographic source:
SETAC Annual Meeting, Nov 2009, New Orleans, LA

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 216 (Soil Microorganisms: Nitrogen Transformation Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
The test article was 97.64% DBDPEthane.

Sampling and analysis

Analytical monitoring:
no

Test substrate

Vehicle:
no

Test organisms

Test organisms (inoculum):
soil

Study design

Total exposure duration:
28 d

Test conditions

Test temperature:
18.1 to 20.8 degrees C
Moisture:
43.0-54.1% of Water Holding Capcity
Nominal and measured concentrations:
0, 2500 mg DBDPEthane/kg dry soil, 500 mg positive control/kg dry soil
Reference substance (positive control):
yes
Remarks:
2 -chloro-6 -(trichloromethyl) pyridine

Results and discussion

Effect concentrations
Duration:
28 d
Dose descriptor:
NOEC
Effect conc.:
2 500 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
nitrate formation rate
Details on results:
The mean nitrate formation rates were 6.1 mg No3/kg dry soil/d in untreated controls, 5.8 mg/kg/d in soils treated with DBDPEthane adn 0.3 mg/kg/d in soils treated with the positive control substances. Soils treated with 2500 mg/kg DBDPEthane showed 5.9% inhibtion compared with untreated controls. Soils treated with 500 mg/kg positive control substance showed 95.0% inhibition in the rate of nitrate formation compared to controls.
Results with reference substance (positive control):
The mean nitrate formation rates were 6.1 mg No3/kg dry soil/d in untreated controls, 5.8 mg/kg/d in soils treated with DBDPEthane adn 0.3 mg/kg/d in soils treated with the positive control substances. Soils treated with 2500 mg/kg DBDPEthane showed 5.9% inhibtion compared with untreated controls. Soils treated with 500 mg/kg positive control substance showed 95.0% inhibition in the rate of nitrate formation compared to controls.

Any other information on results incl. tables

Treatment

Soil Nitrate (mg NO3/kg dry soil)

Day 0

Day 28

Inhibition (%)

Control

26.9

199

---

DBDPEthane

(2500 mg/kg dry soil)

26.0

188

5.6

Reference Inhibitor

(500 mg/kg dry soil)

24.3

32.8

83.5

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
DBDPEthane did not have any long-term adverse effects on soil nitrogen transformation.
Executive summary:

Soil treated with DBDPEthane at a nominal concentration of 2500 mg/kg exhibited approximately 6% inhibition in the rate of nitrate formation after 28 days, compared with untreated control soil; therefore, the test substance did not have any long-term adverse effects on nitrogen transformation.