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Environmental fate & pathways

Biodegradation in water: screening tests

Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Oct 17, 1990 to Jan 10, 1991
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: study performed using international guidelines under GLPs using commercial material as test article and published.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1991
Reference Type:
publication
Title:
Environmental Risk Evaluation Report: 1,1'-(Ethane-1,2-diyl)bis[pentabromobenzene] (CAS no. 84852-53-9)
Author:
Dungey SM, Akintoye L
Year:
2007
Bibliographic source:
http://publications.environment-agency.gov.uk/epages/eapublications.storefront/48771dd8022bcfe8273fc0a8029606a9/Search/Run

Materials and methods

Test guideline
Qualifier:
according to
Guideline:
OECD Guideline 301 C (Ready Biodegradability: Modified MITI Test (I))
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
96.3% decabromodiphenyl ethane, 3.6% dodecabromodiphenyl ethane

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge (adaptation not specified)
Details on inoculum:
300 ml of basal culture medium to which innoculum added so that suspended solid reached 30 mg/L
Duration of test (contact time):
28 d
Initial test substance concentration
Initial conc.:
100 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimationopen allclose all
Parameter followed for biodegradation estimation:
O2 consumption
Parameter followed for biodegradation estimation:
test mat. analysis
Details on study design:
Water + test substance; Sludge + test substance; Sludge + test substance; Sludge + test substance; Sludge + aniline; Control Blank.

Test substance = 100 mg/L. Aniline = 100 mg/L. Activated sludge = 30 mg/L. Temperature 25 +- 1 degree C. Duration 28 d. Measured BOD at 7, 14, 21 and 28 days after innoculation. After incubation, weighed samples and evaluated IR spectrum.
Reference substance
Reference substance:
aniline

Results and discussion

Test performance:
Aniline was degraded 63, 81, 84 and 85% by days 7, 14, 21, and 28, respectively.
% Degradationopen allclose all
Key result
Parameter:
% degradation (O2 consumption)
Value:
0
Sampling time:
28 d
Key result
Parameter:
% degradation (test mat. analysis)
Value:
2
Sampling time:
28 d
Details on results:
Appearance of Test Media
Day 0 Water + test substance : Test substance not dissolved.
Day 0 Sludge + test substance: Test substance not dissolved.
Day 28 Water + test substance: Test substance remained insoluable.
Dau 28 Sludge + test substance: Test substance remained insoluable. No growth of sludge observed

% Biodegradation
Method Percentage biodegradation (%)
Test Vessel 1 Test Vessel 2 Test Vessel 3 Mean
BOD 1 0 0 0
Weight 6 0 1 2


BOD5 / COD results

Results with reference substance:
Aniline was degraded 63, 81, 84 and 85% by days 7, 14, 21, and 28, respectively. The control blank performed as expected. No degradation was seen in the water + test substance vessel.

Any other information on results incl. tables

Table 1. Calculation Table on the Degree of Biodegradation by BOD

Test No. 11893.  Duration of cultivation: 28 days

Vessel

No.

Day 7

Day 14

Day 21

Day 28

Mean

Degr.

(%)

BOD

(5)

Degr.

(%)

BOD

(5)

Degr.

(%)

BOD

(5)

Degr.

(%)

BOD

(5)

Degr.

(%)

1

58.0

63

74.9

81

79.2

87

81.2

85

 

2

0.8

-

1.4

-

2.9

-

4.1

-

 

4

0.8

0

1.2

0

2.5

0

4.2

1

0

5

0.4

0

0.8

0

1.9

0

3.2

0

 

6

0.0

0

0.0

0

1.4

0

2.9

0

 

3

0.0

-

0.0

-

0.0

-

0.0

-

 

 

Degr. : Degree of biodegradation

Vessel No. 1: Sludge + Aniline

Vessel No. 2: Control blank (B)

Vessels No. 4,5,6: Sludge + Test substance

Vessel No. 3: Water + Test Substance

 

Test substance added: 30 mg

Degr. = (BOD-B)/(TOD) × 100%

 

TOD of test substance: 14.7 mg

C14H4Br10+ 15 O2 --> 14 CO2+ 2 H2O + 10 Br

15 O2/ C14H4Br10 = 479.98/971.23 = 0.49

TOD = 30.0 × 0.49 = 14.7 mg

 

TOD of aniline: 90.3 mg

C6H7N + 8.75 O2 -- > 6 CO2+ 3.5 H2O + NO2

8.75 O2/ C6H7N = 279.99/93.13 = 3.01

TOD = 30.0 × 3.01 = 90.3 mg

 

Table 2. Calculation Table for Percentage Biodegradation

Test No. 11893

Vessel No.

Sample description

A

(mg)

D

(mg)

E

(%)

F

(%)

3

Water + Test Substance

28.7

29.8

 

 

4

Sludge + Test Substance

32.0

28.2

6

 

5

Sludge + Test Substance

34.5

30.8

0

2

6

Sludge + Test Substance

33.2

29.4

1

 

2

Control blank

4.9

 

 

 

 

Amount of test substance added: 30 mg

A:   Amount of residue (mg)

B:   Ratio of portion used for analysis: Water (300/300), Sludge (300/300)

C:   Recovery rat: Water (96.2%), Sludge (96.2%)

D:   Residual amount of test substance (mg)

           Water: D=A/B/(C/100)

            Sludge: D=(A-A(Control blank))/B/(C/100)

E:   Percentage biodegradation (%)

           E=(D(Water+ Test Substance)-D(Sludge + Test Substance))/D(Water + Test Substance) × 100

F:   Average percentage biodegradation (%)

Table 3. Calculation Table for the Recovery Rate

Test No. 11893

Sample description

A

(mg)

C

(mg)

D

(%)

E

(%)

Water + Test Substance 1

29.1

29.1

97.0

 

Water + Test Substance 2

28.6

28.6

95.3

96.2

Sludge + Test Substance 1

37.2

28.5

95.0

 

Sludge + Test Substance 2

37.9

29.2

97.3

96.2

Control blank

8.7

 

 

 

Amount of test substance added: 30 mg

A:   Amount of residue (mg)
B:   Ratio of portion used for analysis (300/300)

C:   Recovery amount (mg)

           Water: C=A/B

           Sludge: C=(A-A(Control blank))/B

D:   Recovery rate (%)

           D=C/30 mg × 100

E:   Average recovery rate (%)

DBDPEthane was not readily biodegradable by activated sewage sludge over a 28-day period when tested under Japanese MITI/OECD Ready Biodegradability 301C Modified MITI guidelines.  IR spectra indicated the test substance was unchanged.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed
Conclusions:
DBDPEthane was not readily biodegradable by activated sewage sludge over a 28-day period when tested under Japanese MITI/OECD Ready Biodegradability 301C Modified MITI guidelines.  IR spectra indicated the test substance was unchanged.
Executive summary:

DBDPEthane was not readily biodegradable by activated sewage sludge over a 28-day period when tested under Japanese MITI/OECD Ready Biodegradability 301C Modified MITI guidelines.  IR spectra indicated the test substance was unchanged.