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Ecotoxicological information

Short-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: study performed according to international guidelines and GLPs using commercial product as test article and published in the literature.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
2003
Reference Type:
publication
Title:
Studies and evaluation of the potential toxicity of decabromodiphenyl ethane to five aquatic and sediment organisms.
Author:
Hardy et al.
Year:
2012
Bibliographic source:
Ecotoxicol Environ Saf 75(1):73-9.
Reference Type:
publication
Title:
Unnamed
Year:
2009
Reference Type:
publication
Title:
Unnamed
Year:
2007
Reference Type:
publication
Title:
Unnamed
Year:
2000
Reference Type:
publication
Title:
Unnamed
Year:
1985
Reference Type:
publication
Title:
Unnamed
Year:
1994

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Deviations:
no
Qualifier:
according to
Guideline:
EPA OPP 72-2 (Aquatic Invertebrate Acute Toxicity Test)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid: particulate/powder
Remarks:
migrated information: powder
Details on test material:
98.34 % Decabromodiphenyl ethane
1.66 % Nonabromodiphenyl ethane

Sampling and analysis

Analytical monitoring:
no
Details on sampling:
The study was performed using the Water Accomadated Fraction (WAF). The WAF methodology was chosen due to DBDPEthanes’ negligible water solubility and the difficulty associated with generation and analysis of stable water concentrations in the preliminary work performed for this study.

Test solutions

Vehicle:
no
Details on test solutions:
Preparation and appearance of the test solutions was as for the trout study except that each WAF consisted of 4.2 L of well water.

Test organisms

Test organisms (species):
Daphnia magna
Details on test organisms:
see below

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
no

Test conditions

Hardness:
see below
Test temperature:
see below
pH:
see below
Dissolved oxygen:
see below
Salinity:
see below
Nominal and measured concentrations:
Nominal water accommodated fraction (WAF) loading rates of 0 (well water), 6.9, 14, 28, 55 and 110 mg/L under static conditions. The WAF methodology was chosen due to DBDPEthanes’ negligible water solubility and the difficulty associated with generation and analysis of stable water concentrations in preliminary work.
Details on test conditions:
In the daphnia 48-h study, measured water temperatures were within the 20 ± 2°C range established for the test. Dissolved oxygen concentrations remained at or above 8.2 mg/L 91% of saturation) throughout the test while pH ranged from 8.3 to 8.6. Day 0 dilution water specific conductance (μmhos/cm), hardness (mg/L as CaCO3), alkalinity (mg/L as CaCO3) and total organic carbon (mg C/L) were 310, 124, 184, and <1, respectively.
Reference substance (positive control):
no

Results and discussion

Effect concentrations
Duration:
48 h
Dose descriptor:
EL50
Effect conc.:
>= 110 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
All daphnids in the negative control appeared healthy and normal throughout the test. No immobile or dead organisms were observed n any of the treatment groups after 48 h. The ELR50 values at 24 and 48 h were determined to be >110 mg/L, the highest concentration tested.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
The test article was not acutely toxic to Daphnia magna.
Executive summary:

In the daphnia 48-h study, measured water temperatures were within the 20 ± 2°C range established for the test. Dissolved oxygen concentrations remained at or above 8.2 mg/L 91% of saturation) throughout the test while pH ranged from 8.3 to 8.6. Day 0 dilution water specific conductance (μmhos/cm), hardness (mg/L as CaCO3), alkalinity (mg/L as CaCO3) and total organic carbon (mg C/L) were 310, 124, 184, and <1, respectively. All daphnids in the negative control appeared healthy and normal throughout the test. No immobile or dead organisms were observed in any of the treatment groups after 48 h. The ELR50 values at 24 and 48 h were determined to be >110 mg/L, the highest concentration tested.