Registration Dossier

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Currently viewing:

Administrative data

Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
Aug 19, 2003 to Sept 9, 2003
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: Study performed according to international guidelines and GLPs using commercial product at test article, and results published in the literature.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
2003
Reference Type:
publication
Title:
Studies and evaluation of the potential toxicity of decabromodiphenyl ethane to five aquatic and sediment organisms.
Author:
Hardy et al.
Year:
2012
Bibliographic source:
Ecotoxicol Environ Saf 75(1):73-9.
Reference Type:
publication
Title:
Results of terrestrial and aquatic studies on the brominated flame retardant, decabromodipehnyl ethane, a.k.a. Ethane 1,2 bis(pentaromophenyl)
Author:
Hardy et al.
Year:
2009
Bibliographic source:
SETAC Annual Meeting, 2009, Nov 2009, New Orleans, LA
Reference Type:
publication
Title:
Unnamed
Year:
2007
Reference Type:
publication
Title:
Unnamed
Year:
2000
Reference Type:
publication
Title:
Unnamed
Year:
1985
Reference Type:
publication
Title:
Unnamed
Year:
1994

Materials and methods

Test guidelineopen allclose all
Qualifier:
according to
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
Deviations:
no
Qualifier:
according to
Guideline:
EU Method C.3 (Algal Inhibition test)
Deviations:
no
Qualifier:
according to
Guideline:
EPA OPPTS 850.5400 (Algal Toxicity, Tiers I and II)
Deviations:
no
GLP compliance:
yes

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
98.34 % Decabromodiphenyl ethane
1.66 % Nonabromodiphenyl ethane

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no
Details on test solutions:
see below

Test organisms

Test organisms (species):
Scenedesmus capricornutum
Details on test organisms:
see below

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
no

Test conditions

Hardness:
see below
Test temperature:
see below
pH:
see below
Dissolved oxygen:
see below
Salinity:
see below
Nominal and measured concentrations:
0 (well water), 6.9, 14, 28, 55 and 110 mg/L
Details on test conditions:
see below
Reference substance (positive control):
no

Results and discussion

Effect concentrationsopen allclose all
Duration:
96 h
Dose descriptor:
EL50
Effect conc.:
110 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: growth, density
Duration:
96 h
Dose descriptor:
NOELR
Effect conc.:
110 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: growth, density
Details on results:
In the algae study, measured water temperatures were within the 24 ± 2°C range established for the test. Dissolved oxygen concentrations remained at or above 8.2 mg/L 91% of saturation) throughout the test. The pH of test solutions tended to increase relative to increases in algal densities, which is typical for tests conducted with S. capricornutum. Test solutions ranged from 7.7 to 7.8 at initiation to 9.5 to 10.1 at exposure termination. The light intensity was within the desired range of 4300 ± 10% lux. Changes in cell densities were evaluated over 96-hours, and were used to calculate areas under the growth curve (biomass) and growth rates for each 24-h interval. After 72 and 96 h of exposure, no apparent treatment-related effects on growth were observed at any of the loading rates tested. No significant differences between the negative control and treated groups’ growth were detected (p>0.05). The 72 and 96-h EL50, EbL50 and ErL50 values based on cell density, area under the growth curve and growth rate, respective, were >110 mg/L, the highest tested loading rate. The 72 and 96 h NOAELR for each parameter was 110 mg/L.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
After 72 and 96 h of exposure, no apparent treatment-related effects on growth were observed at any of the loading rates tested. No significant differences between the negative control and treated groups’ growth were detected (p>0.05). The 72 and 96-h EL50, EbL50 and ErL50 values based on cell density, area under the growth curve and growth rate, respective, were >110 mg/L, the highest tested loading rate. The 72 and 96 h NOAELR for each parameter was 110 mg/L.
Executive summary:

In the algae study, measured water temperatures were within the 24 ± 2°C range established for the test. Dissolved oxygen concentrations remained at or above 8.2 mg/L 91% of saturation) throughout the test. The pH of test solutions tended to increase relative to increases in algal densities, which is typical for tests conducted with S. capricornutum. Test solutions ranged from 7.7 to 7.8 at initiation to 9.5 to 10.1 at exposure termination. The light intensity was within the desired range of 4300 ± 10% lux. Changes in cell densities were evaluated over 96-hours, and were used to calculate areas under the growth curve (biomass) and growth rates for each 24-h interval. After 72 and 96 h of exposure, no apparent treatment-related effects on growth were observed at any of the loading rates tested. No significant differences between the negative control and treated groups’ growth were detected (p>0.05). The 72 and 96-h EL50, EbL50 and ErL50 values based on cell density, area under the growth curve and growth rate, respective, were >110 mg/L, the highest tested loading rate. The 72 and 96 h NOAELR for each parameter was 110 mg/L.