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EC number: 263-058-8
CAS number: 61789-40-0
Table 1: Mutagenic activity of test
Revertant colonies/ plate
-b: Not tested
Gene mutation study was performed on Salmonella
Typhimurium strains to determine the mutagenic nature of test
chemical. Salmonella Typhimurium TA 1538, 1535, 1537, 98, 100 and
E. coli WP2 were used during the study. The study was performed in the
presence and absence of S9 metabolic activation. The S9 fraction was
prepared from the liver of male Sprague-Dawley rats, weighing about 220g
(seven weeks old), pretreated with polychlorinated biphenyl (KC 500) at
a dose of 500mg/kg body weight five days before sacrifice. The positive
controls used were 4-nitroquinoline-N-oxide, 9-aminoacridine,
2-nitrofluorene, N-ethyl-N-nitro-N-nitrosoguanidine, benzo(a)pyrene,
2-(2-Furyl)-3-(5-nitro-2-furyl) acrylamide (AF-2) and 9-aminoacridine.
0.1ml of bacterial strain and 0.5ml of S9 mix or sodium phosphate buffer
(pH 7.4) were added to a sterile test tube containing 0.1ml of various
concentrations of the chemical. The test concentrations used were 1, 5,
10, 50, 100, 500, 1000 and 5000 µg/plate and the test chemical was
dissolved in DMSO. This mixture was preincubated in a shaker water bath
at 37°C for 20 minutes, and then added to 2ml of molten top agar (45°C).
This mixture was preincubated in a shaker water bath at 37°C for 20
minutes, and then added to 2ml of molten top agar (45°C). The contents
of each tube were mixed and poured onto a minimal glucose agar plate
immediately. The plates were incubated at 37°C for 48 hours, and then
the number of revertant colonies on each plate was scored with an
automated colony counter. The background bacterial lawn was checked
routinely by dissected microscope. Negleble amount of revertant colonies
were observed as compared to positive control substances. Thus the test
chemical can be considered as non-mutagenic when Salmonella
Typhimurium TA 1538, 1535, 1537, 98, 100 and E. coli WP2 were
treated in the presence and absence of S9 activation system.
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