Registration Dossier

Ecotoxicological information

Short-term toxicity to aquatic invertebrates

Currently viewing:

Administrative data

Link to relevant study record(s)

Reference
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from handbook
Qualifier:
according to
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
Principles of method if other than guideline:
To evaluate short term toxicity of aquatic invertebrate for the test chemical 1-Propanaminium, 3- amino-N-(carboxymethyl)-N,N-dimethyl-, N-coco acyl derivatives, inner salt
GLP compliance:
not specified
Specific details on test material used for the study:
1-Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-coco acyl derivs., hydroxides, inner salts/61789-40-0 / 263-058-8- IUPAC Name: 1-Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-coco acyl derivs., hydroxides, inner salts- Common Name: Cocamidopropyl Betaine- Smiles: CCCCCCCCCCCC(=O)NCCCN{+} CC(=O)O{-}
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Daphnia magna
Test type:
static
Water media type:
other: De chlorinated water
Limit test:
yes
Total exposure duration:
48 h
Post exposure observation period:
observations were made at 1 , 24 and 48 h after the start of treatment
Test temperature:
(20 ± 2 °C)
pH:
8.33 – 8.49
Dissolved oxygen:
67 – 100%.
Nominal and measured concentrations:
0 (control), 0.5, 1, 2, 4, 8 and 16 mg/l
Details on test conditions:
No. of organisms per vessel: 20
Reference substance (positive control):
not specified
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
6.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The product caused 0% immobilization at the 0.5 mg/l concentration. At concentrations of 1 mg/l and 2 mg/l, 2 and 3 daphnia, respectively, out of a total of 40, were found immobile. The 4 mg/l and 8 mg/l concentrations caused immobilization in 5 and 9 daphnia, respectively, out of a total of 20. All the animals treated at the 16 mg/l concentration were found immobile. 1.7% immobilization was recorded in the Control group.
Validity criteria fulfilled:
not specified
Conclusions:
The effect concentration at which 50% immobilization of daphnia was observed to be 6.40 mg/l
Executive summary:

1-Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-coco acyl derivs., hydroxides, inner salts was used as a test material to evaluate toxicity of aquatic invertebrate in a 48 h short term study as described in OECD guideline 202.

 

The product was administered in a single dose per dose level, dissolved in the water held in the test tubes, which was similar to that used in the breeding period. The daphnia were then transferred to the vessels. The dilution water was aerated to saturation level prior to introduction of the test substance to ensure that the oxygen level did not fall below 60% of the saturation value.

 The Daphnia were monitored one, 24 and 48 hours after the start of treatment. Observations included a determination of the number of immobilized animals, that is to say animals not able to swim within 15 seconds after gentle agitation of the test container.

 The product caused 0% immobilization at the 0.5 mg/l concentration. At concentrations of 1 mg/l and 2 mg/l, 2 and 3 daphnia, respectively, out of a total of 40, were found immobile. The 4 mg/l and 8 mg/l concentrations caused immobilization in 5 and 9 daphnia, respectively, out of a total of 20. All the animals treated at the 16 mg/l concentration were found immobile. 1.7% immobilization was recorded in the Control group.

The effect concentration at which 50% immobilization of daphnia was observed to be 6.40 mg/l.

Though , according to the above concentration the test material can be considered as toxic to aquatic environment and can be classified as aquatic chronic– 2 , but the substance is readily biodegradable in aquatic environment. Hence, the test material can not be classified as per CLP classification

Description of key information

Short term toxicity to aquatic invertebrate:

1-Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-coco acyl derivs., hydroxides, inner salts was used as a test material to evaluate toxicity of aquatic invertebrate in a 48 h short term study as described in OECD guideline 202.

 

The product was administered in a single dose per dose level, dissolved in the water held in the test tubes, which was similar to that used in the breeding period. The daphnia were then transferred to the vessels. The dilution water was aerated to saturation level prior to introduction of the test substance to ensure that the oxygen level did not fall below 60% of the saturation value.

 The Daphnia were monitored one, 24 and 48 hours after the start of treatment. Observations included a determination of the number of immobilized animals, that is to say animals not able to swim within 15 seconds after gentle agitation of the test container.

 The product caused 0% immobilization at the 0.5 mg/l concentration. At concentrations of 1 mg/l and 2 mg/l, 2 and 3 daphnia, respectively, out of a total of 40, were found immobile. The 4 mg/l and 8 mg/l concentrations caused immobilization in 5 and 9 daphnia, respectively, out of a total of 20. All the animals treated at the 16 mg/l concentration were found immobile. 1.7% immobilization was recorded in the Control group.

The effect concentration at which 50% immobilization of daphnia was observed to be 6.40 mg/l.

Though , according to the above concentration the test material can be considered as toxic to aquatic environment and can be classified as aquatic chronic– 2 , but the substance is readily biodegradable in aquatic environment. Hence, the test material can not be classified as per CLP classification

Key value for chemical safety assessment

EC50/LC50 for freshwater invertebrates:
6.4 mg/L

Additional information

Short term toxicity to aquatic invertebrate:

The available data for test chemical 1-Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-coco acyl derivs., hydroxides, inner salts from peer reviewed handbook , authorative databases are summarized below.

1) 1-Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-coco acyl derivs., hydroxides, inner salts was used as a test material to evaluate toxicity of aquatic invertebrate in a 48 h short term study as described in OECD guideline 202.

 

The product was administered in a single dose per dose level, dissolved in the water held in the test tubes, which was similar to that used in the breeding period. The daphnia were then transferred to the vessels. The dilution water was aerated to saturation level prior to introduction of the test substance to ensure that the oxygen level did not fall below 60% of the saturation value.

 The Daphnia were monitored one, 24 and 48 hours after the start of treatment. Observations included a determination of the number of immobilized animals, that is to say animals not able to swim within 15 seconds after gentle agitation of the test container.

 The product caused 0% immobilization at the 0.5 mg/l concentration. At concentrations of 1 mg/l and 2 mg/l, 2 and 3 daphnia, respectively, out of a total of 40, were found immobile. The 4 mg/l and 8 mg/l concentrations caused immobilization in 5 and 9 daphnia, respectively, out of a total of 20. All the animals treated at the 16 mg/l concentration were found immobile. 1.7% immobilization was recorded in the Control group.

The effect concentration at which 50% immobilization of daphnia was observed to be 6.40 mg/l.

Though , according to the above concentration the test material can be considered as toxic to aquatic environment and can be classified as aquatic chronic– 2 , but the substance is readily biodegradable in aquatic environment. Hence, the test material can not be classified as per CLP classification.

2) Short term toxicity of test material Cocamidopropyl Betaine was evaluated on Daphnia magna for 48h according to OECD guideline 202.The daphnids were exposed to the substance in 50-ml beakers containing 20 ml of test solution with 10 daphnids per beaker.Daphnids were laboratory bred and less than 24 hours old at test initiation. Test levels were 0 (control), 6.25, 12.5, 25, 50 and 100 mg/l. Test levels were run in duplicate The EC50 for the test chemical on aquatic invertebrate after 48 hrs was observed to be 21.5 mg/l.

Though , according to the above concentration the test material can be considered as toxic to aquatic environment and can be classified as aquatic chronic– 2 , but the substance is readily biodegradable in aquatic environment. Hence, the test material can not be classified as per CLP classification