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Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from handbook
Qualifier:
according to
Guideline:
other: as mentioned below
Principles of method if other than guideline:
To evaluate short term toxicity of fish for the test chemical 1 Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-(C12-18(even numbered) acyl) derivs., hydroxides, inner salts.
GLP compliance:
not specified
Specific details on test material used for the study:
1-Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-coco acyl derivs., hydroxides, inner salts/61789-40-0 / 263-058-8- IUPAC Name: 1-Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-coco acyl derivs., hydroxides, inner salts- Common Name: Cocamidopropyl Betaine- Smiles: CCCCCCCCCCCC(=O)NCCCN{+} CC(=O)O{-} -The test substance purity was reportedly 30% active ingredient
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
- Common name: Zebrafish
Test type:
static
Water media type:
freshwater
Total exposure duration:
96 h
Nominal and measured concentrations:
0, 5.66 or 8.0 mg/L
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
LC0
Effect conc.:
1.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
96 h
Dose descriptor:
LC100
Effect conc.:
2.4 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Validity criteria fulfilled:
not specified
Conclusions:
The median lethal concentration (EC50) for test material on Zebrafish after 96 h was observed to be 2.0 mg/l
Executive summary:

1 Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-(C12-18(even numbered) acyl) derivs., hydroxides, inner salts was used as a test material to evaluate its toxicity to fish Zebrafish (Brachydanio rerio) , the fish were exposed to nominal concentrations of 0 , 5.66 or 8.0 mg/l under static condition for 96 h The test substance purity was reported 30% active ingredient. All fish exposed to 8.0 mg/L died within the first 24 hours of exposure.

 

The medial lethal concentration (EC50) for test material on Zebrafish after 96 h was observed to be 2.0 mg/l.

 

Though , according to the above concentration the test material can be considered as toxic to aquatic environment and can be classified as acute aquatic – 2 , but the substance is readily biodegradable in aquatic environment. Hence, the test material can not be classified as per CLP classification

Description of key information

Short term toxicity to fish:

1 Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-(C12-18(even numbered) acyl) derivs., hydroxides, inner salts was used as a test material to evaluate its toxicity to fish Zebrafish (Brachydanio rerio) , the fish were exposed to nominal concentrations of 0 , 5.66 or 8.0 mg/l under static condition for 96 h The test substance purity was reported 30% active ingredient. All fish exposed to 8.0 mg/L died within the first 24 hours of exposure.

 

The medial lethal concentration (EC50) for test material on Zebrafish after 96 h was observed to be 2.0 mg/l.

 

Though , according to the above concentration the test material can be considered as toxic to aquatic environment and can be classified as acute aquatic – 2 , but the substance is readily biodegradable in aquatic environment. Hence, the test material can not be classified as per CLP classification

Key value for chemical safety assessment

LC50 for freshwater fish:
2 mg/L

Additional information

Short term toxicity to fish:

The available data for test chemical 1-Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-coco acyl derivs., hydroxides, inner salts from peer reviewed handbook , authorative databases are summarized below.

1) 1 Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-(C12-18(even numbered) acyl) derivs., hydroxides, inner salts was used as a test material to evaluate its toxicity to fish Zebrafish (Brachydanio rerio) , the fish were exposed to nominal concentrations of 0 , 5.66 or 8.0 mg/l under static condition for 96 h The test substance purity was reported 30% active ingredient. All fish exposed to 8.0 mg/L died within the first 24 hours of exposure.

 

The medial lethal concentration (EC50) for test material on Zebrafish after 96 h was observed to be 2.0 mg/l.

 

Though , according to the above concentration the test material can be considered as toxic to aquatic environment and can be classified as acute aquatic – 2 , but the substance is readily biodegradable in aquatic environment. Hence, the test material can not be classified as per CLP classification.

2) Short term test to evaluate toxicity of test material1-Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-coco acyl derivs., hydroxides, inner salts was performed according to OECD Guideline 203.

 

For the main study, two groups were used, each containing 10 animals. Ten Control fish were also included. Animals were placed in 84-liter glass tanks

containing 50 liters of de chlorinated drinking water.

 

The 10 fish exposed to the 8.0 mg/l concentration died during the first 24 hours after treatment. Before death, they showed a decrease in mobility and breathing difficulties. Two of the animals remained on the surface of the aquarium and the others at the bottom. The 5.66 mg/l concentration did not cause the death of any of the treated animals. Only a slight decrease in mobility was noted between 3 and 24 hours after treatment. None of the fish in the Control group died. The medial lethal effect concentration was observed to be 6.73 mg/l after 96 hrs of treatment.

Though , according to the above concentration the test material can be considered as toxic to aquatic environment and can be classified as acute aquatic – 2 , but the substance is readily biodegradable in aquatic environment. Hence, the test material can not be classified as per CLP classification.

3)The test subastance1-Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-coco acyl derivatives, inner salt (betaine) was evaluated for its short term toxicity effect on Cyprinus carpio.

The nominal test concentrtaions used in the test were 0, 1.0, 1.7, 3.0, 5.0, and 9 mg/L. under semi static conditions for 96 hours. The test substance purity was reportedly 29.6% active ingredient. The median lethal concentration of test material on Cyprinus carpio was observed to be 1.9 mg/l after 96 h.

Though , according to the above concentration the test material can be considered as toxic to aquatic environment and can be classified as acute aquatic – 2 , but the substance is readily biodegradable in aquatic environment. Hence, the test material can not be classified as per CLP classification