1-Propanaminium, 3-amino-N-(carboxymethyl)-N,N-dimethyl-, N-coco acyl derivs., hydroxides, inner salts

Short-term toxicity to fish

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to fish

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Data is from handbook

Qualifier:

according to guideline

Guideline:

other: as mentioned below

Principles of method if other than guideline:

The objective of the study was to evaluate short term toxicity of test chemical to Danio rerio for 96 h.

GLP compliance:

not specified

Analytical monitoring:

not specified

Vehicle:

not specified

Test organisms (species):

Danio rerio (previous name: Brachydanio rerio)

Details on test organisms:

- Common name: Zebrafish

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Nominal and measured concentrations:

0, 5.66 or 8.0 mg/L

Reference substance (positive control):

not specified

Duration:

96 h

Dose descriptor:

LC0

Effect conc.:

1.7 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Key result

Duration:

96 h

Dose descriptor:

LC50

Effect conc.:

2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Duration:

96 h

Dose descriptor:

LC100

Effect conc.:

2.4 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mortality (fish)

Details on results:

Study was conducted to determine effect of test chemical to fish. The median lethal concentration (EC50) for test material on Zebrafish after 96 h was observed to be 2.0 mg/l.

Validity criteria fulfilled:

not specified

Conclusions:

Study was conducted to determine effect of test chemical to fish. The median lethal concentration (EC50) for test material on Zebrafish after 96 h was observed to be 2.0 mg/l.

Executive summary:

An experiment was conducted to determine the short term toxicity of test chemical to fish. In this study test organism used was Danio rerio (previous name: Brachydanio rerio). The nominal concentrations of test chemical used were of 0, 5.66 or 8.0 mg/l. The test chemical purity was reported to be 30% active ingredient The experiment was conducted in static condition and test chemical was exposed to fish for 96 h. All fish exposed to 8.0 mg/L died within the first 24 hours of exposure. The medial lethal concentration (EC50) for test chemical on Danio rerio after 96 h was observed to be 2.0 mg/l. Since the test chemical is readily biodegradable in nature test chemical is considered to be non toxic and cannot be Classified as per CLP regulation.

Description of key information

An experiment was conducted to determine the short term toxicity of test chemical to fish. In this study test organism used was Danio rerio (previous name: Brachydanio rerio). The nominal concentrations of test chemical used were of 0, 5.66 or 8.0 mg/l. The test chemical purity was reported to be 30% active ingredient The experiment was conducted in static condition and test chemical was exposed to fish for 96 h. All fish exposed to 8.0 mg/L died within the first 24 hours of exposure. The medial lethal concentration (EC50) for test chemical on Danio rerio after 96 h was observed to be 2.0 mg/l. Since the test chemical is readily biodegradable in nature test chemical is considered to be non toxic and cannot be Classified as per CLP regulation.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish

Effect concentration:

2 mg/L

Additional information

Various studies have been conducted to determine the toxicity of test chemical to fish from different sources and their results are summarized below. 

In first study an experiment was conducted to determine the short term toxicity of test chemical to fish. In this study test organism used was Danio rerio (previous name: Brachydanio rerio). The nominal concentrations of test chemical used were of 0, 5.66 or 8.0 mg/l. The test chemical purity was reported to be 30% active ingredient The experiment was conducted in static condition and test chemical was exposed to fish for 96 h. All fish exposed to 8.0 mg/L died within the first 24 hours of exposure. The medial lethal concentration (EC50) for test chemical on Danio rerio after 96 h was observed to be 2.0 mg/l. 

 

In another study the experiment was conducted to determine the short term toxicity of test chemical to fish by using OECD Guideline 203 (Fish, Acute Toxicity Test). In this study test organism used was Danio rerio (previous name: Brachydanio rerio) whose Length at study initiation of study was 2.4 and 3.0 cm long and Acclimation period was of 12 days. In study two groups were made, each containing 10 animals. Ten Control fish were also included. Animals were placed in 84-liter glass tanks containing 50 liters of de chlorinated drinking water.

After exposure of test chemical of concentration 8.0 mg/l to fish 100% mortality was observed during the first 24 hours. Before death, they showed a decrease in mobility and breathing difficulties. Two of the animals remained on the surface of the aquarium and the others at the bottom. The 5.66 mg/l concentration did not cause the death of any of the treated animals. Only a slight decrease in mobility was noted between 3 and 24 hours after treatment. No mortality was observed in the control group. The medial lethal effect concentration was observed to be 6.73 mg/l after 96 h of exposure. 

 

The last experimental study was conducted to determine the short term toxicity of test chemical. In this study test organism used was Cyprinus carpio. The active ingredient of test chemical was reported29.6% .The nominal concentrations of test chemical used were 0, 1.0, 1.7, 3.0, 5.0, and 9 mg/L. The experiment was conducted under semi-static condition for 96 h. The median lethal concentration (LC 50 value) of test chemical on Cyprinus carpio was observed to be 1.9 mg/l after 96 h.

 

By considering results of all the studies mentioned above the LC50 value was determined to be in range 2.0 mg/L to 6.73 mg/l. Since the test chemical is readily biodegradable in nature test chemical is considered to be non toxic and cannot be classified as per CLP regulation.