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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms, other
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
data from handbook or collection of data
Justification for type of information:
Data is from handbook
Qualifier:
according to guideline
Guideline:
other: as mentioned below
Principles of method if other than guideline:
Toxicity of test chemical on microorganism was evaluated
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
not specified
Test organisms (species):
Pseudomonas putida
Test type:
static
Water media type:
freshwater
Total exposure duration:
16 h
Duration:
16 h
Dose descriptor:
EC0
Effect conc.:
> 3 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: cell multiplication inhibition
Duration:
30 min
Dose descriptor:
EC0
Effect conc.:
> 3 000 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
other: oxygen consumption inhibition
Validity criteria fulfilled:
not specified
Conclusions:
The effect concentration at which no effect was observed was determined to be >3000 mg/l on the basis of both effects cell multiplication inhibition and oxygen consumption inhibition after exposure of test chemical for 16 h.
Executive summary:

To determine the toxicity of test chemical on microorganism experiment was conducted, in the microorganism used was Pseudomonas putida. The efficiency in cell multiplication and oxygen consumption inhibition of test organism was observed. The effect concentration at which no effect was observed was determined to be >3000 mg/l  on the basis of both effects cell multiplication inhibition and oxygen consumption inhibition after exposure of test chemical for 16 h.

Description of key information

Toxicity to microorganism:

To determine the toxicity of test chemical on microorganism experiment was conducted, in the microorganism used was Pseudomonas putida. The efficiency in cell multiplication and oxygen consumption inhibition of test organism was observed.The effect concentration at which no effect was observed was determined to be >3000 mg/l  on the basis of both effects cell multiplication inhibition and oxygen consumption inhibition after exposure of test chemical for 16 h.

Key value for chemical safety assessment

EC50 for microorganisms:
3 000 mg/L

Additional information

Toxicity to microorganism:

Different studies have been conducted to determine the toxicity of test chemical to microorganism and their results are summarized below.                                                                                   

 

The first study was conducted to determine the toxicity of test chemical on microorganism, in this the microorganism used was Pseudomonas putida. The efficiency in cell multiplication and oxygen consumption inhibition of test organism was observed. The effect concentration at which no effect was observed was determined to be >3000 mg/l after exposure of test chemical for 16 h.

 

In another study the experiment was conducted to determine the toxicity of test chemical to microorganisms. The test organisms used were Pseudomonas aeruginosa, Staphylococcus epidermis, Bacillus albicans and Aspergillus niger. The test chemical was exposed to these microorganisms for 24 h. The concentrations at which no effects (EC0) were observed was determined to be>100 mg/L onPseudomonas aeruginosa. The concentration of test chemical at which 10% inhibitions (EC10) occurs was determined to be 2.5mg/L on Staphylococcus epidermis and 20 mg/L on Bacillus albicans and Aspergillus niger.

 

By considering results of both the studies mentioned above the EC0 value was determined to be in range >100 mg/L to 3000 mg/L.