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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: inhalation

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Administrative data

Endpoint:
acute toxicity: inhalation
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Adopted according to OECD SIDS (public available peer reviewed source). The original source is not available and has not been reviewed.

Data source

Referenceopen allclose all

Reference Type:
review article or handbook
Title:
1,4-Dichlorobut-2-ene- CAS No: 764-41-0
Author:
OECD SIDS
Year:
2006
Bibliographic source:
SIDS Initial Assessment Report for 22th SIAM, UNEP Publications
Reference Type:
study report
Title:
Unnamed
Year:
1974
Report date:
1974

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The LD50 of a mixture of 1.43 % cis- and 97.17% trans-isomer has been determined in ChR-CD rats after a 30 minutes exposure.
GLP compliance:
no
Remarks:
GLP was not mandatory at the time of the study
Test type:
standard acute method
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
1,4-dichlorobut-2-ene
EC Number:
212-121-8
EC Name:
1,4-dichlorobut-2-ene
Cas Number:
764-41-0
Molecular formula:
C4H6Cl2
IUPAC Name:
1,4-dichlorobut-2-ene
Details on test material:
1.43 % cis- and 97.17% trans-1,4-dichlorobut-2-ene.

Test animals

Species:
rat
Strain:
other: ChR-CD
Sex:
male

Administration / exposure

Route of administration:
inhalation: vapour
Type of inhalation exposure:
whole body
Vehicle:
other: filtered air
Analytical verification of test atmosphere concentrations:
not specified
Duration of exposure:
30 min
Concentrations:
240/370/410/440/540/760/2600 ppm
No. of animals per sex per dose:
6 (only male rats)
Control animals:
other: not required
Details on study design:
- Duration of observation period following administration: 14 days
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, histopathology, gross examination. Gross and histopathologic examinations were performed on 2 rats surviving exposure for 7 days, on 1 rat found dead after 13 recovery days and on 1 rat after 14 recovery days.
Statistics:
LC50 calculations were made by a computerized version of the probit method of Finney (1964).

Results and discussion

Effect levels
Sex:
male
Dose descriptor:
LC50
Effect level:
4.1 mg/L air
Based on:
test mat.
Exp. duration:
30 min
Mortality:
Mortality %: 0/0/33/0/0 /83/100
Clinical signs:
other: Clinical signs were observed already in the lowest dose group (240 ppm; 1248 mg/m³): Inactivity, closed eyes, shallow respiration, pale ears, salivation, lacrimation, wet fur and reversible loss of body weight. Clinical signs at 760 ppm: Irregular respira
Body weight:
No data
Gross pathology:
No data
Other findings:
- Histopathology: at 410 ppm and above: damage of the tracheobronchial epithelium was observed. Furthermore in animals exposed to 760 ppm (3950 mg/m³) there was kidney damage, testicular atrophy and hypoplastic bone marrow observed. These effects have been interpreted by the authors of the study as representing only a reflection of stress and emaciation and thus being not directly compound-related. The other tissues examined (lymph node, stomach, duodenum, epididymis, thyroid, adrenal gland, brain, eye) revealed no effects being attributable to the test substance.

Applicant's summary and conclusion