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Diss Factsheets

Environmental fate & pathways

Biodegradation in water: screening tests

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Administrative data

Endpoint:
biodegradation in water: ready biodegradability
Type of information:
experimental study
Adequacy of study:
other information
Study period:
2004-10-22 to 2004-11-23
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
other: GLP guideline study

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
2004
Report date:
2005
Reference Type:
secondary source
Title:
1,4-Dichlorobut-2-ene - CAS No: 764-41-0
Author:
OECD SIDS
Year:
2006
Bibliographic source:
SIDS Initial Assessment Report for 22th SIAM, UNEP Publications

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
EU Method C.4-E (Determination of the "Ready" Biodegradability - Closed Bottle Test)
Version / remarks:
Cited as Directive 92/69/EEC, C.4-E
Principles of method if other than guideline:
In all essential parts identical with OECD Guideline 301 D
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Reference substance name:
Automatically generated during migration to IUCLID 6, no data available
IUPAC Name:
Automatically generated during migration to IUCLID 6, no data available
Details on test material:
IUCLID4 Test substance: other TS: 1,4-Dichlorobut-2-ene, purity 96.1% (31.9% cis- and 64.2% trans-isomer)

Study design

Oxygen conditions:
aerobic
Inoculum or test system:
activated sludge, domestic, non-adapted
Details on inoculum:
Mixed population of aquatic organism from the secondary effluent of a domestic sewage treatment plant (Wupper area water authority, Germany), separation of coarse particles by filtration, aeration for 4 days - 5 mL/L effluent concentration in reaction mixture - pH values: No data documented (according to SOP pH 7.4 at start of incubation)
Initial test substance concentration
Initial conc.:
5.5 mg/L
Based on:
test mat.
Parameter followed for biodegradation estimation
Parameter followed for biodegradation estimation:
other: BOD
Details on study design:
Exposure condition: 28 days incubation at 22 +/- 2 °C
Reference substance
Reference substance:
other: sodium benzoate

Results and discussion

% Degradation
Parameter:
other: BOD
Value:
20
Sampling time:
28 d
Details on results:
Kinetic of test substance (in %):
0 after 7 day(s)
0 after 14 day(s)
3 after 21 day(s)

Any other information on results incl. tables

All validity criteria of the test method were met.
Theoretical oxygen demand (ThOD) of test substance: 1280 mg O2/g; pH  value was 7.4 at begin and 7.0 at end of test period.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Interpretation of results:
under test conditions no biodegradation observed