Registration Dossier
Registration Dossier
Data platform availability banner - registered substances factsheets
Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.
The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 931-313-9 | CAS number: -
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Repeated dose toxicity: inhalation
Administrative data
- Endpoint:
- short-term repeated dose toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Only secondary literature. However, according to OECD SIDS a reliability of 2 was given.
Data source
Referenceopen allclose all
- Reference Type:
- publication
- Title:
- Toxicity of chloroprene, 1,3-dichlorobutene-2, and 1,4-dichlorobutene-2
- Author:
- Clary JJ
- Year:
- 1 977
- Bibliographic source:
- Environm. Health Perspect 21, 269-274.
- Reference Type:
- secondary source
- Title:
- 1,4-Dichlorobut-2-ene - CAS No: 764-41-0
- Author:
- OECD SIDS
- Year:
- 2 006
- Bibliographic source:
- SIDS Initial Assessment Report for 22th SIAM, UNEP Publications.
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- 6 animals/group/strain were tested; 3/6 were sacrificed 1 day after the last exposure, while 3/6 were sacrificed after a 14 d-recovery period.
- GLP compliance:
- no
- Remarks:
- GLP was not mandatory at the time of the study
Test material
- Reference substance name:
- 1,4-dichlorobut-2-ene
- EC Number:
- 212-121-8
- EC Name:
- 1,4-dichlorobut-2-ene
- Cas Number:
- 764-41-0
- Molecular formula:
- C4H6Cl2
- IUPAC Name:
- 1,4-dichlorobut-2-ene
- Details on test material:
- Test substance: low boilers 0.07% (weight%)
3,4-DCB-1 0.15%
cis 1,4-DCB-2 11.14%
trans 1,4-DCB-2 86.86%
cis 1,3,4-TCB-2 0.96%
trans 1,3,4-TCB-2 0.49%
other high boilers 0.30%
DCB= dichlorobutene, TCB= trichlorobutene.
Constituent 1
Test animals
- Species:
- rat
- Strain:
- other: CHR-CD and Long Evans (LE)
- Sex:
- male
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- whole body
- Details on inhalation exposure:
- Vapour generation: liquid 1,4-dichlorobut-2-ene was delivered at a constant rate by a syringe pump into a heated (ca. 110°C) round-bottom flask. Air metered into the flask carried the resulting vapours into a glass 30-liter chamber containing the test animals.
- Analytical verification of doses or concentrations:
- yes
- Details on analytical verification of doses or concentrations:
- Chamber atmosphere was examined in 1h-intervals by gas chromatography (electron capture detector):
0.11 +/- 0.05 ppm
10.9 +/- 2.5 ppm - Duration of treatment / exposure:
- 2 weeks
- Frequency of treatment:
- 6 h/day, 5 days/week
Doses / concentrations
- Remarks:
- Doses / Concentrations:
0.1, 10 ppm = 0.00052, 0.052 mg/L
Basis:
nominal conc.
- No. of animals per sex per dose:
- 6 (only male rat tested)
- Control animals:
- yes
- Details on study design:
- Post-exposure period: 2 weeks.
- Positive control:
- No
Examinations
- Observations and examinations performed and frequency:
- No data
- Sacrifice and pathology:
- GROSS PATHOLOGY: Yes. At autopsy the following organs were weighed: Heart, liver, kidney, spleen, thymus and testes.
HISTOPATHOLOGY: Yes. The following organs and tissues were examined microscopically: Trachea, lung, heart, liver, esophagus, stomach, intestine, pancreas, thyroid, parathyroid, adrenal glands, bone marrow, lymph node, spleen, thymus, brain, eye and skin.
Results and discussion
Results of examinations
- Clinical signs:
- effects observed, treatment-related
- Mortality:
- mortality observed, treatment-related
- Body weight and weight changes:
- effects observed, treatment-related
- Food consumption and compound intake (if feeding study):
- not examined
- Food efficiency:
- not examined
- Water consumption and compound intake (if drinking water study):
- not examined
- Ophthalmological findings:
- not examined
- Haematological findings:
- not examined
- Clinical biochemistry findings:
- not examined
- Urinalysis findings:
- not examined
- Behaviour (functional findings):
- not examined
- Organ weight findings including organ / body weight ratios:
- not specified
- Gross pathological findings:
- effects observed, treatment-related
- Description (incidence and severity):
- at 0.052 mg/L, reversible
- Histopathological findings: non-neoplastic:
- effects observed, treatment-related
- Description (incidence and severity):
- at 0.052 mg/L, reversible
- Histopathological findings: neoplastic:
- not examined
- Details on results:
- 0.00052 mg/L: No effect
0.052 mg/L: reduced weight gain; 1 day after last exposure 2/3 (CHR) and 1/3 (LE) showed growth retardation with concomitant atrophy of the liver, kidneys, spleen, thymus, testes and adrenal glands but without any histopathological alteration. These effects were reversible within the 14 days post-observation period. In most rats an inflammation of the tracheobronchial area was observed, including, in most rats, epithelial changes such as flattening, squamous metaplasia, hyperplasia, hypertrophy, denudation, and vesiculation. The severity decreased gradually from the upper respiratory tract to the lower portion. There was also a slight acute inflammatory response characterized by dilation of the lumen which contained some exudate consisting of mucus, cells with abundant vesiculated cytoplasm (perhaps due to glycogen accumulation), and a few neutrophils.
These microscopic changes were reversible within the 14-day recovery period except for some luminal exudate still present in a few rats.
Effect levels
open allclose all
- Dose descriptor:
- NOAEC
- Effect level:
- 0.001 other: mg/L
- Based on:
- test mat.
- Sex:
- male
- Dose descriptor:
- LOAEC
- Effect level:
- 0.052 other: mg/L
- Based on:
- test mat.
- Sex:
- male
- Basis for effect level:
- other: Reduced body weight gain and inflammation of the respiratory tract
Target system / organ toxicity
- Critical effects observed:
- not specified
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.