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Toxicological information

Acute Toxicity: oral

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Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Adopted according to OECD SIDS (public available peer reviewed source). The original source is available and has been reviewed.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1959
Report Date:
1959
Reference Type:
secondary source
Title:
1,4-Dichlorobut-2-ene- CAS: 764-41-0
Author:
OECD SIDS
Year:
2006
Bibliographic source:
SIDS Initial Assessment Report for 22th SIAM, UNEP Publications

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
In an acute oral toxicity study on male albino rats (3-5 animals/group) the LD50 has been determined.
GLP compliance:
no
Remarks:
GLP was not mandatory at the time of the study
Test type:
standard acute method

Test material

Reference
Name:
Unnamed
Type:
Constituent
Details on test material:
- Name of test material (as cited in study report): 1,4-dichlorobut-2-ene.
- Analytical purity: no data

Test animals

Species:
rat
Strain:
other: albino
Sex:
male

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: Oil
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 0.05, 0.10, 0.25, 0.50 mL/kg bw
- Amount of vehicle (if gavage): between 1180-1190 mL
- Justification for choice of vehicle: test substance undergoes hydrolysis in water

MAXIMUM DOSE VOLUME APPLIED: between 1180-1190 mL

Doses:
59, 119, 297, 593 mg/kg bw
No. of animals per sex per dose:
3-5
Control animals:
other: not necessary

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LD50
Effect level:
> 120 - 300 mg/kg bw
Sex:
male
Dose descriptor:
other: NOAEL
Effect level:
59 mg/kg bw
Sex:
male
Dose descriptor:
other: LOAEL
Effect level:
119 mg/kg bw
Remarks on result:
other: LOAEL yield a mortality of 20 % (1/5 animals) and toxic symptoms like spasms, inappetence and weakness were observed in all treated animals. At 297 mg/kg bw 66% mortality (2/3 animals) was observed.
Mortality:
59 mg/kg bw (0.05 mL/kg bw 0.5 % solution in oil): no mortality NOAEL (n= 3);
119 mg/kg bw (0.10 mL/kg bw 1.0 % solution in oil): mortality 1/5;
297 mg/kg bw (0.25 mL/kg bw 2.5 % solution in oil): mortality 2/3;
593 mg/kg bw (0.50 mL/kg bw 5.0 % solution in oil): mortality 3/3.
Clinical signs:
119 mg/kg bw (0.10 mL/kg bw 1.0 % solution in oil): all animals with toxic symptoms (spasms, inappetence, weakness).
297 mg/kg bw (0.25 mL/kg bw 2.5 % solution in oil): all animals with toxic symptoms (spasms, inappetence, weakness).
593 mg/kg bw (0.50 mL/kg bw 5.0 % solution in oil): all animals with toxic symptoms (spasms, inappetence, weakness).
Body weight:
no data
Gross pathology:
no data

Applicant's summary and conclusion