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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Acute Toxicity: oral

Currently viewing:

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Adopted according to OECD SIDS (public available peer reviewed source). The original source is available and has been reviewed.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1959
Report date:
1959
Reference Type:
secondary source
Title:
1,4-Dichlorobut-2-ene- CAS: 764-41-0
Author:
OECD SIDS
Year:
2006
Bibliographic source:
SIDS Initial Assessment Report for 22th SIAM, UNEP Publications

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
In an acute oral toxicity study on male albino rats (3-5 animals/group) the LD50 has been determined.
GLP compliance:
no
Remarks:
GLP was not mandatory at the time of the study
Test type:
standard acute method

Test material

Constituent 1
Chemical structure
Reference substance name:
1,4-dichlorobut-2-ene
EC Number:
212-121-8
EC Name:
1,4-dichlorobut-2-ene
Cas Number:
764-41-0
Molecular formula:
C4H6Cl2
IUPAC Name:
1,4-dichlorobut-2-ene
Details on test material:
- Name of test material (as cited in study report): 1,4-dichlorobut-2-ene.
- Analytical purity: no data

Test animals

Species:
rat
Strain:
other: albino
Sex:
male

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
other: Oil
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 0.05, 0.10, 0.25, 0.50 mL/kg bw
- Amount of vehicle (if gavage): between 1180-1190 mL
- Justification for choice of vehicle: test substance undergoes hydrolysis in water

MAXIMUM DOSE VOLUME APPLIED: between 1180-1190 mL

Doses:
59, 119, 297, 593 mg/kg bw
No. of animals per sex per dose:
3-5
Control animals:
other: not necessary

Results and discussion

Effect levelsopen allclose all
Sex:
male
Dose descriptor:
LD50
Effect level:
> 120 - 300 mg/kg bw
Sex:
male
Dose descriptor:
other: NOAEL
Effect level:
59 mg/kg bw
Sex:
male
Dose descriptor:
other: LOAEL
Effect level:
119 mg/kg bw
Remarks on result:
other: LOAEL yield a mortality of 20 % (1/5 animals) and toxic symptoms like spasms, inappetence and weakness were observed in all treated animals. At 297 mg/kg bw 66% mortality (2/3 animals) was observed.
Mortality:
59 mg/kg bw (0.05 mL/kg bw 0.5 % solution in oil): no mortality NOAEL (n= 3);
119 mg/kg bw (0.10 mL/kg bw 1.0 % solution in oil): mortality 1/5;
297 mg/kg bw (0.25 mL/kg bw 2.5 % solution in oil): mortality 2/3;
593 mg/kg bw (0.50 mL/kg bw 5.0 % solution in oil): mortality 3/3.
Clinical signs:
119 mg/kg bw (0.10 mL/kg bw 1.0 % solution in oil): all animals with toxic symptoms (spasms, inappetence, weakness).
297 mg/kg bw (0.25 mL/kg bw 2.5 % solution in oil): all animals with toxic symptoms (spasms, inappetence, weakness).
593 mg/kg bw (0.50 mL/kg bw 5.0 % solution in oil): all animals with toxic symptoms (spasms, inappetence, weakness).
Body weight:
no data
Gross pathology:
no data

Applicant's summary and conclusion