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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in mammalian cells
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1998

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
The ability of lead chloride (0.1 to 1.0 uM) to induce H202 formation in Chinese hamster ovary AS52 cells and the relationship between H202 induction and mutagenicity were examined.
GLP compliance:
not specified
Type of assay:
other: mammalian cell gene mutation assay

Test material

Reference
Name:
Unnamed
Type:
Constituent
Test material form:
solid
Details on test material:
CAS number not specified
Specific details on test material used for the study:
CAS number not specified

Results and discussion

Test results
Species / strain:
Chinese hamster Ovary (CHO)
Metabolic activation:
not specified
Genotoxicity:
positive
Cytotoxicity / choice of top concentrations:
cytotoxicity
Vehicle controls validity:
valid
Untreated negative controls validity:
not examined
Positive controls validity:
not examined
Remarks on result:
other: Test system: all strains/cell types tested

Any other information on results incl. tables

Exposure to lead chloride at 0.8 and 1.0 uM increased H202 induction and mutation frequency in AS52 cells. Addition of allopurinol (an inhibitor of H202 induction) reduced the mutation frequency in lead-treated cells, implicating H202 induction in mutagenesis.

Applicant's summary and conclusion

Conclusions:
Interpretation of results: positive
Executive summary:

The ability of lead chloride (0.1 to 1.0 uM) to induce H202 formation in Chinese hamster ovary AS52 cells and the relationship between H202 induction and mutagenicity were examined. Exposure to lead chloride at 0.8 and 1.0 uM increased H202 induction and mutation frequency in AS52 cells. Addition of allopurinol (an inhibitor of H202 induction) reduced the mutation frequency in lead-treated cells, implicating H202 induction in mutagenesis.