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Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Reference
Endpoint:
toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Remarks:
Original report is not available. However, the report is cited by a peer-reviewed OECD SIDS. Hence, the reliability was taken from the OECD report.
Qualifier:
according to guideline
Guideline:
OECD Guideline 201 (Alga, Growth Inhibition Test)
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Scenedesmus capricornutum
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
72 h
Hardness:
30.3 mg/L
Test temperature:
22.8 - 23.2 °C
pH:
9.03 (start of the test); 7.90 (end of the test)
Dissolved oxygen:
9.13 - 9.25 mg/L
Nominal and measured concentrations:
Test concentrations (nominal): 10, 18, 32, 56 and 100 mg/L
Details on test conditions:
TEST SYSTEM
- Test vessel:
- Material, size, headspace, fill volume: 100 mL medium in a 300 mL conical flask.
- Initial cells density: 10 000 cell/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3


GROWTH MEDIUM
- Standard medium used: yes (OECD Medium)


OTHER TEST CONDITIONS
- Light intensity and quality: 4000 - 5000 lux
- Photoperiod: 24h light per day
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
41.6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
biomass
Remarks on result:
other: 95% C.I. = 37.3 - 46.5 mg/L
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
69.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth rate
Remarks on result:
other: 95% C.I. = 57.1 - 85.1 mg/L
Key result
Duration:
72 h
Dose descriptor:
NOEC
Effect conc.:
32 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.

Measured concentrations of MADAME:

Nominal conc. (mg/L)

Measured conc. (mg/L)

24 h

% of nominal

72 h

% of nominal

Control

N.D.

-

N.D.

-

10

8.82

88.2

N.D.

-

18

15.2

84.4

0.07

0.39

32

25.9

80.9

0.30

0.94

56

48.2

86.3

0.86

1.54

100

83.4

83.4

2.18

2.18

Nominal cell density:

Nominal conc. (mg/L)

Cell density (x 10000 cells/mL)

0 h

24 h

48 h

72 h

Control

1.0

3.6

18.7

81.6

10

1.0

3.5

15.2

83.9

18

1.0

4.0

16.3

80.3

32

1.0

2.8

12.8

53.7

56

1.0

2.3

6.6

30.2

100

1.0

1.0

0.361

2.9

Validity criteria fulfilled:
yes

Description of key information

2-dimethylaminoethyl methacrylate is harmful to aquatic algae.

Key value for chemical safety assessment

EC50 for freshwater algae:
41.6 mg/L

Additional information

The determination of inhibitory effects of 2-dimethylaminoethyl methacrylate on Scenedesmus subspicatus was performed in a OECD Guideline 201 study (Alga, Growth Inhibition Test). The results of this study showed a EbC50 of 41.6 mg/L. The measurement of the growth inhibition showed a ErC50 value of 69.7 mg/L. Therefore, with high probability 2-dimethylaminoethyl methacrylate is harmful to aquatic algae.